Abstract

Introduction and Objectives : Applying herbal-acupuncture using Ursi Fel into Zusanli (UZ) on to the atopic dermatitis(AD) in mice to study changes in external dermal formation, change of leukocytes in vasculature, change of lipid formation in stratum corneum and distribution of ceramide; this study is done through forcing an injury to the mice's back skin which damages the lipid protection formation in the stratum corneum. Materials and Methods : The AD which was caused intentionally using the external application on the mice's back skin was treated with UZ; the change of leukocytes in the vasculature had been identified, through optima 5.2 and student's t-test and the results were made into a dermal formation graph. Results : After dispensing UZ into the AD, the dermal injury was decreased. The recovery of the lipid protection formation which includes lipid and ceramide in the stratum corneum(for suppressing acute inflammation due to factors such as PKC, TNF-ɑ, IL-1β, which controled the secretion of the relating inflammatory cytokine) also went onto show a decrease of both angiogenesis and degranulated mast cells. In addition, the decrease of epithelial injury also caused the growth of cells to decrease in the stratum basale and cytoclasis. In the vasculature, the leukocytes were also decreased and this could relate to a decrease in AD. Conclusions : UZ has an effect on AD by suppressing dermal injury through the recovery of the lipid protection formation in the stratum corneum.