- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Antioxidant Property of the Gagam-Hyungbang-Gihwang-tang Using Biochemical Markers of Carcinogenesis
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.3, pp.204-214
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(2005). Antioxidant Property of the Gagam-Hyungbang-Gihwang-tang Using Biochemical Markers of Carcinogenesis. Journal of Korean Medicine, 26(3), 204-214.
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Abstract
Objectives : The verity extract of the Gagam-Hyungbang-Gihwang-tang (GHG) was assessed to determine the mechanisms of its antioxidant activity. Methods : The following effects were measured : GHG exhibited a concentration-treatment; scavenging α,α-diphenyl-β- picrylhydrazyl (DPPH) radical, linoleic acid oxidation in a thiocyanate assay system, and superoxide anion, hydroxyl radicalinduced DNA nicking. We investigated mRNA levels such as superoxide-dismutase. Results : The GHG extract showed dose-dependent free radical scavenging activity, including DPPH radicals, hydroxyl radicals, and superoxide anion, using different systems. The GHG was also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals in Fenton’ s reaction mixture. Furthermore, SOD-1 mRNA expression levels increased in rat hepatoma H4IIE cells. Conclusions : We expect that GHG will be helpful to the development of antioxidant activity treatments.
- keywords
- Hyungbang-Gihwang-tang, antioxidant activity, DPPH, superoxide anion, superoxide dismutase-1
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