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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development and Validation of an LC-MS/MS Method for Determination of Damaurone D in Rat Plasma and its Application to Pharmacokinetic Study in Rats

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.1, pp.38-42
Lee Wonpyo (Dankook University)
Song Im-Sook (Kyungpook National University)
Han Young Taek (Dankook University)
Choi Min-Koo (Dankook University)
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Damaurone D belongs to the genus Rosa and is a traditional medicinal product used for the treatment of depression, inflammation, and infectious diseases. The purpose of this study was to develop a simple liquid chromatography-tandem mass spectrometry method for the detection of damaurone D in rat plasma and to demonstrate its application in pharmacokinetic stud-ies. Damaurone D and berberine (internal standard) were extracted with acetonitrile using a protein precipitation method. Mass transition was monitored in multiple reaction monitoring mode at m/z 323.2→267.0 for damaurone D and m/z 336.1→320.0 for berberine in positive ion mode. Analytical validation was conducted by evaluating the specificity, linearity, accuracy, precision, matrix effect, extraction recovery, and stability. The calibration curves were linear over 2–1000 ng/mL. The intra- and inter-day precision and accuracy of quality control samples were 4.79–13.33% and 86.23–102.75%, respectively. The matrix effect and extraction recovery were 96.11–98.47% and 96.11–102.25%, respectively. In the pharmacokinetic study after intravenous administration of damaurone D at a dose of 3 mg/kg in rats, the area under the curve and clearance of damaurone D in rat plasma were 16750.26 ± 2676.10 min·ng/mL and 182.44 ± 31.36 mL/min/kg, respectively.

Damaurone D, LC-MS/MS, analytical method validation, pharmacokinetic study



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