- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.4, pp.117-122
https://doi.org/10.5478/MSL.2019.10.4.117
(Sogang University)
(Sogang University)
(Sogang University)
(Sogang University)
(Sogang University)
Yu,
S., Jang,
H., &
Oh,
H.
B.
(2019). Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants. Mass Spectrometry Letters, 10(4), 117-122, https://doi.org/10.5478/MSL.2019.10.4.117
- Downloaded
- Viewed
Abstract
This study demonstrated the sensitivity of electron capture dissociation mass spectrometry (ECD-MS) to probe sub- tle conformational changes in gaseous melittin ions induced by the substitution of an amino acid. ECD-MS was performed for triply and quadruply-protonated melittin and its variants obtained by a single amino acid substitution, namely, D-Pro14, Pro14Ala, and Leu13Ala. Although native triply-protonted melittin showed only a few peptide backbone cleavage products, the D-Pro14 and Pro14Ala variants exhibited extensive backbone fragments, suggesting the occurrence of a significant structural or conformational change induced by a single amino acid substitution at Pro14. On the contrary, the substitution at Leu13, namely Leu13Ala (+3), did not cause significant changes in the ECD backbone fragmentation pattern. Thus, the sensitivity of ECD-MS is demonstrated to be good enough to probe the aforementioned conformational change in melittin.
- keywords
- Electron Capture Dissociation, Melittin, Amino Acid Substitution, Chiral Inversion, Mass Spectrometry
Reference
1
Ganem, B.. (1991). . J. Am. Chem. Soc, 113, 6294-.
2
Loo, J. A.. (1997). . Mass Spectrom. Rev, 16, 1-.
3
Oh, H. B.. (2002). . Proc. Natl. Acad. Sci. U. S. A, 99, 15863-.
4
Adams, C. M.. (2004). . J. Am. Soc. Mass Spectrom, 15, 1087-.
5
Breuker, K.. (2008). . Proc. Natl. Acad. Sci. U. S. A, 105, 18145-.
6
Schennach, M.. (2016). . J. Am. Soc. Mass Spectrom, 27, 1079-.
7
Wyttenbach, T.. (2003). . Top. Curr. Chem, 225, 207-.
8
Koeniger, S. L.. (2006). . J. Am. Chem. Soc, 128, 11713-.
9
Ko, J. Y.. (2011). . J. Phys. Chem. A, 115, 14215-.
10
Winger, B. E.. (1992). . J. Am. Chem. Soc, 114, 5897-.
11
McLafferty, F. W.. (1998). . J. Am. Chem. Soc, 120, 4732-.
12
Lee, S .Y.. (2006). . J. Am. Soc. Mass Spectrom, 17, 536-.
13
Lee, S. Y.. (2006). . Rapid Commun. Mass Spectrom, 20, 3167-.
14
Lee, S. Y.. (2009). . Int. J. Mass Spectrom, 279, 47-.
15
Snow, C. D.. (2002). . J. Am. Chem. Soc, 124, 14548-.
16
Carnevail, P.. (2003). . J. Am. Chem. Soc, 125, 14244-.
17
Patriksson, A.. (2007). . J. Phys. Chem. B, 111, 13147-.
18
Kaltashov I. A.. (1997). . Prot. Struct. Func. Gen, 27, 165-.
19
Bazzo, R.. (1988). . Eur. J. Biochem, 173, 139-.
20
Gerig, J. T.. (2004). . Biophys. J, 86, 3166-.
21
Roccatano, D.. (2005). . Prot. Sci, 14, 2582-.
22
Terra, R. M. S.. (2007). . Mol. Grap. Mod, 25, 767-.
23
Florance, H. V.. (2011). . Analyst, 136, 3446-3452.
24
Iavarone, A. T.. (2000). . J. Am. Soc. Mass Spectrom, 11, 976-985.
25
Zubarev, R. A.. (2000). . Anal. Chem, 72, 563-573.
26
Meot-Ner, M. J.. (1983). . J. Am. Chem. Soc, 105, 4906-4911.
27
Shi, L.. (2015). . J. Am. Chem. Soc, 137, 8680-.
28
Hewish, D. R.. (2002). . J. Prot. Chem, 21, 243-.
- Submission Date
- 2019-12-14
- Revised Date
- 2019-12-24
- Accepted Date
- 2019-12-24
Other articles from this issue
- Effect of Ultrasonic Microdroplet Generation in the Low-Temperature Plasma Ionization-Mass Spectrometry
- Hydrolysis of Penicillin G and Carbenicillin in Pure Water - As Studied by HPLC/ESI-MS
- Development of a Chloroform Reference Material for the Proficiency Testing of Hazardous Compounds in Commercial Consumer Chemical Products Under the Consumer Chemical Products and Biocide Safety Management Act (K-BPR)
- Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.