• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.4, pp.117-122
https://doi.org/10.5478/MSL.2019.10.4.117
Yu Seonghyun (Sogang University)
Jang Hwa-yong (Sogang University)
Oh Han Bin (Sogang University)
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Abstract

This study demonstrated the sensitivity of electron capture dissociation mass spectrometry (ECD-MS) to probe sub- tle conformational changes in gaseous melittin ions induced by the substitution of an amino acid. ECD-MS was performed for triply and quadruply-protonated melittin and its variants obtained by a single amino acid substitution, namely, D-Pro14, Pro14Ala, and Leu13Ala. Although native triply-protonted melittin showed only a few peptide backbone cleavage products, the D-Pro14 and Pro14Ala variants exhibited extensive backbone fragments, suggesting the occurrence of a significant structural or conformational change induced by a single amino acid substitution at Pro14. On the contrary, the substitution at Leu13, namely Leu13Ala (+3), did not cause significant changes in the ECD backbone fragmentation pattern. Thus, the sensitivity of ECD-MS is demonstrated to be good enough to probe the aforementioned conformational change in melittin.

keywords
Electron Capture Dissociation Melittin Amino Acid Substitution Chiral Inversion Mass Spectrometry


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Submission Date
2019-12-14
Revised Date
2019-12-24
Accepted Date
2019-12-24
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