- P-ISSN 2233-4203
- E-ISSN 2093-8950
- Log In/Sign Up
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Analysis of Hypoxia-Inducible Factor Stabilizers by a Modified QuEChERS Extraction for Antidoping Analysis
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2020, v.11 no.4, pp.118-124
https://doi.org/10.5478/MSL.2020.11.4.118
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Sogang University)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Sogang University)
(Korea Institute of Science and Technology)
Kim,
S. H., Lim,
N. R., Min,
H., Sung,
C., Oh,
H. B., &
Kim,
K.
H.
(2020). Analysis of Hypoxia-Inducible Factor Stabilizers by a Modified QuEChERS Extraction for Antidoping Analysis. Mass Spectrometry Letters, 11(4), 118-124, https://doi.org/10.5478/MSL.2020.11.4.118
- Downloaded
- Viewed
Abstract
An analytical method was developed for hypoxia-inducible factor (HIF) stabilizers based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation and liquid chromatography–high resolution mass spectrometry analysis. HIF stabilizers potentially enhance the performance of athletes, and hence, they have been prohibited. However, the analysis of urinary HIF stabilizers is not easy owing to their unique structure and characteristics. Hence, we developed the QuEChERS preparation technique for a complementary method and optimized the pH, volume of extraction solvent, and number of extractions. We found that double extraction with 1% of formic acid in acetonitrile provided the highest recovery of HIF stabilizers. Moreover, the composi-tion of the mobile phase was also optimized for better separation of molidustat and IOX4. The developed method was validated in terms of its precision, detection limit, matrix effect, and recovery for ISO accreditation. To the best of our knowledge, this is the first demonstration of the application of the QuEChERS method, which is suitable as a complementary analytical method, in antidoping.
- keywords
- antidoping, hypoxia-inducible factor stabilizer, QuEChERS
- Submission Date
- 2020-12-18
- Revised Date
- 2020-12-30
- Accepted Date
- 2020-12-30
Other articles from this issue
- Advances in Ion Mobility Spectrometry-Mass Spectrometry (IMS-MS)-Based Techniques for Elucidating Higher-Order Protein Structures
- A Simple and Efficient Method to Determine Montelukast in Rat Plasma Using Liquid-Liquid Extraction and Tandem Mass Spectrometry
- Comparison of Matrices for Optimal Analysis of Synthetic Polymers Using MALDI-TOF Mass Spectrometry
- Simultaneous Determination of Anthraquinone, Flavonoids, and Phenolic Antidiabetic Compounds from Cassia auriculata Seeds by Validated UHPLC Based MS/MS Method
- Characterization of Natural Organic Matter in Spring Water
- A Low-Cost Open-Source Air Sampler for the Sorbent Tube Sampling for TD-GC/MS Analysis
- Chiral Derivatization of Hydroxycarboxylic Acids Using 2,4,6-Trichlorobenzoyl Chloride as a Highly Efficient Regioselective Esterification Reagent for Gas Chromatography-Mass Spectrometry
- Development of a Mushroom Powder Certified Reference Material for Element Analysis
- Inhibitory Potential of Bilobetin Against CYP2J2 Activities in Human Liver Microsomes
- Analysis of Hypoxia-Inducible Factor Stabilizers by a Modified QuEChERS Extraction for Antidoping Analysis
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.