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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.137-145
Lee Hyun Hee L. (Korea Food Research Institute)
Chae Soo Yeon (Korea University)
Son Myung Kook (Korea University)
Kim Hugh I. (Korea University)
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Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS com-bined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-con-taining peptide ions.

ion mobility mass spectrometry, tryptic peptide, ion chemistry, glutamine

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