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- E-ISSN 2093-8950
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Identification of HYIpro-3-1 Metabolites, a Novel Anti-Inflammatory Compound, in Human Liver Microsomes by Quadrupole-Orbitrap High-Resolution Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.172-178
https://doi.org/10.5478/MSL.2021.12.4.172
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Yeungnam University)
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Yeungnam University)
(Kyungpook National University)
Bai,
H., Kim,
Y., Paudel,
S., Lee,
E., &
Lee,
S.
(2021). Identification of HYIpro-3-1 Metabolites, a Novel Anti-Inflammatory Compound, in Human Liver Microsomes by Quadrupole-Orbitrap High-Resolution Mass Spectrometry. Mass Spectrometry Letters, 12(4), 172-178, https://doi.org/10.5478/MSL.2021.12.4.172
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Abstract
HYIpro-3-1 is an adjuvant for preventing or treating inflammatory growth diseases. In this study, we identified the metabolic pathway of HYIpro-3-1 in human liver microsomes (HLMs) by quadrupole-orbitrap high-resolution mass spectrometry (HR-MS) and characterized the major human cytochrome P450 (CYP). Ten metabolites were identified, including one O-demethylation (M1), two O-demethylation and monohydroxylation (M2 and M3), and seven monohydroxylation metabolites (M4–M10). Based on the HR-MS 2 spectra, the metabolites are divided into two groups of monohydroxylated metabolites according to the hydroxylation position. We verified that HYIpro-3-1 is metabolized by CYP in HLMs, CYP2B6 is mainly involved in O-demethylation, and various CYPs are involved in the monohydroxylation of HYIpro-3-1.
- keywords
- HYIpro-3-1, LC-HR/MS, cytochrome P450, human liver microsomes
- Submission Date
- 2021-10-13
- Revised Date
- 2021-11-23
- Accepted Date
- 2021-11-24
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