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- E-ISSN 2093-8950
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Development of a Sensitive Analytical Method of Polynemoraline C Using LC-MS/MS and Its Application to a Pharmacokinetic Study in Mice
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.200-205
https://doi.org/10.5478/MSL.2021.12.4.200
(Dankook University)
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Dankook University)
(Dankook University)
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Dankook University)
(Dankook University)
Pang,
M., Lee,
J., Jeon,
J., Song,
I., Han,
Y. T., &
Choi,
M.
(2021). Development of a Sensitive Analytical Method of Polynemoraline C Using LC-MS/MS and Its Application to a Pharmacokinetic Study in Mice. Mass Spectrometry Letters, 12(4), 200-205, https://doi.org/10.5478/MSL.2021.12.4.200
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Abstract
Polynemoraline C, a pyridocoumarin alkaloid, exhibits anticholinergic, anti-inflammatory, antitumor, and antimicrobial activities. A sensitive analytical method of polynemoraline C in mouse plasma was developed and validated using liquid chro-matography-tandem mass spectrometry (LC-MS/MS). Polynemoraline C and 13 C-caffeine (internal standard) in mouse plasma were extracted using a liquid-liquid extraction method coupled with ethyl acetate. This extraction method resulted in high and reproducible extraction recovery in the range of 73.49%-77.31% with no interfering peaks around the peak retention time of polynemoraline C and 13 C-caffeine. The standard calibration curves for polynemoraline C were linear over the range of 0.5-200 ng/mL with r 2 > 0.985. The accuracy, precision, and the stability of the data were within acceptable limits on the FDA guideline. After intravenous and oral administration of polynemoraline C at doses of 5 and 30 mg/kg, respectively, the present method was successfully applied to the pharmacokinetic study of polynemoraline C. Polynemoraline C in mouse plasma showed a multi-exponential elimination pattern with a high volume of distribution values. This compound’s absolute oral bioavailability was found to be 17.0%. Polynemoraline C’s newly developed LC-MS/MS method can be used for further studies on the efficacy, toxicity, and biopharmaceutics of polynemoraline C, as well as its pharmacokinetic studies.
- keywords
- Polynemoraline C, LC-MS/MS analysis, Pharmacokinetics, Bioavailability
- Submission Date
- 2021-10-12
- Revised Date
- 2021-11-28
- Accepted Date
- 2021-12-13
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