- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Identification of HYIpro-3-1 Metabolites, a Novel Anti-Inflammatory Compound, in Human Liver Microsomes by Quadrupole-Orbitrap High-Resolution Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.172-178
https://doi.org/10.5478/MSL.2021.12.4.172
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Yeungnam University)
(Kyungpook National University)
(Kyungpook National University)
(Kyungpook National University)
(Yeungnam University)
(Kyungpook National University)
Bai,
H., Kim,
Y., Paudel,
S., Lee,
E., &
Lee,
S.
(2021). Identification of HYIpro-3-1 Metabolites, a Novel Anti-Inflammatory Compound, in Human Liver Microsomes by Quadrupole-Orbitrap High-Resolution Mass Spectrometry. Mass Spectrometry Letters, 12(4), 172-178, https://doi.org/10.5478/MSL.2021.12.4.172
- Downloaded
- Viewed
Abstract
HYIpro-3-1 is an adjuvant for preventing or treating inflammatory growth diseases. In this study, we identified the metabolic pathway of HYIpro-3-1 in human liver microsomes (HLMs) by quadrupole-orbitrap high-resolution mass spectrometry (HR-MS) and characterized the major human cytochrome P450 (CYP). Ten metabolites were identified, including one O-demethylation (M1), two O-demethylation and monohydroxylation (M2 and M3), and seven monohydroxylation metabolites (M4–M10). Based on the HR-MS 2 spectra, the metabolites are divided into two groups of monohydroxylated metabolites according to the hydroxylation position. We verified that HYIpro-3-1 is metabolized by CYP in HLMs, CYP2B6 is mainly involved in O-demethylation, and various CYPs are involved in the monohydroxylation of HYIpro-3-1.
- keywords
- HYIpro-3-1, LC-HR/MS, cytochrome P450, human liver microsomes
- Submission Date
- 2021-10-13
- Revised Date
- 2021-11-23
- Accepted Date
- 2021-11-24
Other articles from this issue
- Special Issue in Honor of Prof. Seung Koo Shin
- Looking through the Mass-to-Charge Ratio: Past, Present and Future Perspectives
- Integrated Thermochemical Approach to Collision-Induced Dissociation Process of Peptides
- Ion Mobility Signatures of Glutamine-Containing Tryptic Peptides in the Gas Phase
- Elucidating H/D-Exchange Mechanism of Active Hydrogen in Aniline and Benzene-1,2-dithiol
- Li+ and Li+I−Li+ ions Solvated by 1,4-dioxane: An ion Mobility Spectrometry-Mass Spectrometry Study
- Effect of Cation Complexation of Hindered Phenol Antioxidants on their Fragmentation in Electrospray Ionization Tandem Mass Spectrometry
- LC-MS/MS-based Proteomic Analysis to Identify Protein Phosphorylation in Emiliania huxleyi
- Identification of HYIpro-3-1 Metabolites, a Novel Anti-Inflammatory Compound, in Human Liver Microsomes by Quadrupole-Orbitrap High-Resolution Mass Spectrometry
- Fragmentation Analysis of rIAPP Monomer, Dimer, and [M rIAPP + M hIAPP ]5+ Using Collision-Induced Dissociation with Electrospray Ionization Mass Spectrometry
- Separation of Light Rare-Earth Elements Using Gas-Pressurized Extraction Chromatography
- Molecular Characterization of Dissolved Organic Matter Unveils their Complexity, Origin, and Fate in Glacier and Glacial-Fed Streams and Lakes on the Tibetan Plateau
- Development of a Sensitive Analytical Method of Polynemoraline C Using LC-MS/MS and Its Application to a Pharmacokinetic Study in Mice
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.