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  • P-ISSN2233-4203
  • E-ISSN2093-8950
  • ESCI, SCOPUS, KCI

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development of a Sensitive Analytical Method of Polynemoraline C Using LC-MS/MS and Its Application to a Pharmacokinetic Study in Mice

Development of a Sensitive Analytical Method of Polynemoraline C Using LC-MS/MS and Its Application to a Pharmacokinetic Study in Mice

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2021, v.12 no.4, pp.200-205
https://doi.org/10.5478/MSL.2021.12.4.200
Minyeong Pang (Dankook University)
Jaehyeok Lee (Kyungpook National University)
Ji-Hyeon Jeon (Kyungpook National University)
Im-Sook Song (Kyungpook National University)
Young Taek Han (Dankook University)
Min-Koo Choi (Dankook University)
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Abstract

Polynemoraline C, a pyridocoumarin alkaloid, exhibits anticholinergic, anti-inflammatory, antitumor, and antimicrobial activities. A sensitive analytical method of polynemoraline C in mouse plasma was developed and validated using liquid chro-matography-tandem mass spectrometry (LC-MS/MS). Polynemoraline C and 13 C-caffeine (internal standard) in mouse plasma were extracted using a liquid-liquid extraction method coupled with ethyl acetate. This extraction method resulted in high and reproducible extraction recovery in the range of 73.49%-77.31% with no interfering peaks around the peak retention time of polynemoraline C and 13 C-caffeine. The standard calibration curves for polynemoraline C were linear over the range of 0.5-200 ng/mL with r 2 > 0.985. The accuracy, precision, and the stability of the data were within acceptable limits on the FDA guideline. After intravenous and oral administration of polynemoraline C at doses of 5 and 30 mg/kg, respectively, the present method was successfully applied to the pharmacokinetic study of polynemoraline C. Polynemoraline C in mouse plasma showed a multi-exponential elimination pattern with a high volume of distribution values. This compound’s absolute oral bioavailability was found to be 17.0%. Polynemoraline C’s newly developed LC-MS/MS method can be used for further studies on the efficacy, toxicity, and biopharmaceutics of polynemoraline C, as well as its pharmacokinetic studies.

keywords
Polynemoraline C, LC-MS/MS analysis, Pharmacokinetics, Bioavailability


투고일Submission Date
2021-10-12
수정일Revised Date
2021-11-28
게재확정일Accepted Date
2021-12-13
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