- P-ISSN 2233-4203
- E-ISSN 2093-8950
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
Article Detail
Home > Article Detail
Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.2, pp.33-36
https://doi.org/10.5478/MSL.2011.2.2.033
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Hanyang University)
(Korea Institute of Science and Technology)
(Korea Institute of Science & Technology)
(Inje University)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Hanyang University)
(Korea Institute of Science and Technology)
(Korea Institute of Science & Technology)
(Inje University)
(Korea Institute of Science and Technology)
Lee,
K. M., Kim,
H. J., Jeong,
E. S., Yoo,
H. H., Kwon,
O., Jin,
C., Kim,
D. H., &
Lee,
J.
(2011). Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human. Mass Spectrometry Letters, 2(2), 33-36, https://doi.org/10.5478/MSL.2011.2.2.033
- Downloaded
- Viewed
Abstract
The misuse of anabolic androgenic steroids is of particular concern in sports and society. Thus, it is of great importance todiscriminate endogenous steroids such as testosterone or testosterone prohormones from their chemically identical syntheticcopies. In this study, gas chromatography-combustion/isotope ratio mass spectrometric (GC−C/IRMS) method has been developedand validated for discriminating the origin of anabolic androgenic steroids. The method involves the solid-phase extraction,enzymatic hydrolysis with β-glucuronidase, HPLC-fractionation for the cleanup and analysis by GC−C/IRMS. The difference(Δ13C) of urinary δ13C values between synthetic analogues and endogenous reference compounds (ERC) by GC−C/IRMS wasused to elucidate the origin of steroids, and intra- and inter-day precision, specificity and isotope fractionation were evaluated. The present GC−C/IRMS method combined with HPLC cleanup was accurate and reproducible enough to be successfullyapplied to the test of urine sample from suspected anabolic steroid abusers.
- keywords
- Steroids, Endogenous, Validation, GC-C/IRMS
Reference
1
Cawley, A. T. (2009). . V. Steoids, 74, 379-.
2
The World Anti-Doping Agency. Prohibited List.
3
Chung, B. C. (1990). . J. Anal. Toxicol, 14, 91-.
4
Coppen, J. J. W.. (1979). . Trop. Sci, 21, 125-.
5
Ueki, M. (1999). . Rapid Commun. Mass Spectrom, 13, 2237-.
6
Becchi, M. (1994). . Rapid Commun. Mass Spectrom, 8, 304-.
7
Aguilera, R. (1996). . J. Mass Spectrom, 31, 169-.
8
Shackleton, C. H. L.. (1997). . Steoids, 62, 379-.
9
Cawley, A. T. (2008). . J. Mass Spectrom, 43, 854-.
10
Mareck, U. (2008). . J. Mass Spectrom, 43, 877-.
11
Shackleton, C. H. L. (1997). . Steoids, 62, 665-.
12
Aguilera, R. (1999). . J. Chromatogr. B, 727, 95-.
13
Aguilera, R. (2001). . Clin.Chem, 47, 292-.
14
Piper, T. (2008). . Rapid Commun. Mass Spectrom, 22, 2161-.
15
Saudan, C. (2009). . J.Chromatogr. B, 877, 2321-.
- Submission Date
- 2011-03-03
- Revised Date
- 2011-03-24
- Accepted Date
- 2011-06-08
Other articles from this issue
- Introduction of Taiwan Society for Mass Spectrometry
- Validation and Applications of Gas Chromatography-Combustion/isotope Ratio Mass Spectrometric Method to Control Misuse of Androgens in Human
- Accurate Quantification of Saccharin Using Isotope Dilution Liquid Chromatography Mass Spectrometry (ID-LC/MS)
- Compositional Characterization of Petroleum Heavy Oils Generated from Vacuum Distillation and Catalytic Cracking by Positive-mode APPI FT-ICR Mass Spectrometry
- Effective Analysis of Dicyclanil in Lamb and Chicken Muscle using HPLCUV/ Vis and LC/MS/MS
- Mass spectrometric studies of competitive binding of C60 and C70 to mesosubstituted porphyrins
- Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis
- Determination of the Uranium Backgrounds in Lexan Films for Single Particle Analysis using FT-TIMS technique
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.