• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.1, pp.15-17
https://doi.org/10.5478/MSL.2012.3.1.015
Pyo Jaesung (National Forensic Service)
Lee Heesang (National Forensic Service)
Park Yujin (National Forensic Service)
Jo Jiyeong (National Forensic Service)
Park Yonghoon (National Forensic Service)
Choe Sanggil (National Forensic Service)
Lee Miyoung (Health Research Institute)
Lee Jaesin (National Forensic Service)
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Abstract

Eighteen of the PDE-5 inhibitors and their analogues were analyzed using GC-EI-MS. Fourteen of them could beidentified by simple GC-MS method without derivatization, but hydroxyhongdenafil, hydroxyvardenafil, xanthoanthrafil andmirodenafil could not be identified without derivatization for the high polarity due to the presence of hydroxyl groups. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), widely used trimethylsilyl (TMS) derivatizing reagents, were used to improve the sensitivity of the hydroxylated analogues. And the analytes could be identified by GC-MS after the derivatization.

keywords
PDE-5 inhibitor GC-MS BSTFA MTBSTFA TMS derivatization


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Submission Date
2011-05-02
Revised Date
2012-02-07
Accepted Date
2012-02-23
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