- P-ISSN 2233-4203
- E-ISSN 2093-8950
- 권한신청
- P-ISSN2233-4203
- E-ISSN2093-8950
- ESCI, SCOPUS, KCI
논문 상세
Home > 논문 상세
Microwave-assisted Weak Acid Hydrolysis of Proteins
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.2, pp.47-49
https://doi.org/10.5478/MSL.2012.3.2.47
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
Seo,
M.,
Kim,
J. H.,
Park,
S.,
Lee,
J.,
Kim,
T.,
Lee,
J.,
&
Kim,
J.
(2012). . Mass Spectrometry Letters, 3(2), 47-49, https://doi.org/10.5478/MSL.2012.3.2.47
- 다운로드 수
- 조회수
Abstract
Myoglobin was hydrolyzed by microwave-assisted weak acid hydrolysis with 2% formic acid at 37 oC, 50 oC, and100 oC for 1 h. The most effective hydrolysis was observed at 100 oC. Hydrolysis products were investigated using matrixassistedlaser desorption/ionization time-of-flight mass spectrometry. Most cleavages predominantly occurred at the C-termini ofaspartyl residues. For comparison, weak acid hydrolysis was also performed in boiling water for 20, 40, 60, and 120 min. A 60-min weak acid hydrolysis in boiling water yielded similar results as a 60-min microwave-assisted weak acid hydrolysis at100 oC. These results strongly suggest that microwave irradiation has no notable enhancement effect on acid hydrolysis of proteinsand that temperature is the major factor that determines the effectiveness of weak acid hydrolysis.
- keywords
- Microwave, Mass spectrometry, MALDI, Acid hydrolysis, Aspartic acid cleavage, Trypsin
참고문헌
1
Weber, M. (2005). . Clin. Biochem, 38, 1027-.
2
Gevaert, K. (2000). . Electrophoresis, 21, 1145-.
3
Cottrell, J. S. (1994). . Pept. Res, 7, 115-.
4
Han, K. (1983). . Int. J. Biol. Chem, 15, 875-.
5
Sun, W. (2006). . Mol. Cell Proteomics, 5, 769-.
6
Pramanik, B. N. (2002). . Protein Sci, 11, 2676-.
7
Lin, S. S. (2005). . J. Am. Soc. Mass Spectrom, 16, 581-.
8
Ji Hye Lee. (2011). Microwave-assisted Protein Digestion on Various Locations of a Microplate. Mass Spectrometry Letters, 2(4), 84-87.
9
Zhong, H. (2004). . Nat. Biotechnol, 22, 1291-.
10
Zhong, H. (2005). . J. Am. Soc. Mass Spectrom, 16, 471-.
11
Reiz, B. (2010). . J. Am. Soc. Mass Spectrom, 21, 1596-.
12
Yang, H. J. (2010). . Rapid Commun. Mass Spectrom, 24, 901-.
13
Yang, H. J. (2011). . Rapid Commun. Mass Spectrom, 25, 88-.
14
Shin, S. (2011). . Anal. Biochem, 414, 125-.
15
Inglis, A. S. (1983). . Methods Enzymol, 91, 324-.
16
Li, A. (2001). . Anal. Chem, 73, 5395-.
17
Hua, L. (2006). . Proteomics, 6, 586-.
- 투고일Submission Date
- 2012-05-31
- 수정일Revised Date
- 2012-06-18
- 게재확정일Accepted Date
- 2012-06-18
권호 내 다른 논문
- Gas Chromatography-High Resolution Tandem Mass Spectrometry Using a GC-APPI-LIT Orbitrap for Complex Volatile Compounds Analysis
- An Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography in Phospho- and Glycoproteome Profiling of Epicardial Adipose Tissue in Obesity Mouse
- Application of Comprehensive 2D GC-MS and APPI FT-ICR MS for More Complete Understanding of Chemicals in Diesel Fuel
- Microwave-assisted Weak Acid Hydrolysis of Proteins
- Simultaneous Determination of Synthetic Phosphodiesterase-5 Inhibitors in Dietary Supplements by Liquid Chromatography-High Resolution/Mass Spectrometry
- Isotope Measurement of Uranium at Ultratrace Levels Using Multicollector Inductively Coupled Plasma Mass Spectrometry
- Determination of Mercury in Fly Ash by Using Flow Injection Cold Vapor Isotope Dilution Inductively Coupled Plasma Mass Spectrometry
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.