After success of sildenafil for the treatment of erectile dysfunction, a large number of its analogues have beenapproved from FDA. Recently, the illegal dietary supplements which include sildenafil, vardenafil, tadalafil, or analogues ofthese drugs as ingredient have been widely distributed. Therefore, the determination of the residue of synthetic phosphodiesterase-5 (PDE-5) inhibitors in dietary supplements is highly required due to indiscriminate and unintentional overdose causednausea, chest pains, fainting and irregular heartbeat. In this paper, we report a rapid and sensitive analytical method for thesimultaneous determination of nine phosphodiesterase-5 inhibitors by liquid chromatography-high resolution mass spectrometry. The present method was found to be accurate and reproducible with 40 μg/g of the limit of quantification for the nine PDE-5inhibitors. The developed method can be successfully applied to the analysis of the seven illegal dietary supplements.
Palit, V. (2010). . Nat. Rev. Urol, 7, 603-.
Reepmeyer, J. C. (2007). . J. Pharmaceut. Biomed, 44, 887-.
Reepmeyer, J. C. (2009). . J. Pharmaceut. Biomed, 49, 145-.
Hou, P. L. (2006). . Food Addit. Contam, 23, 870-.
Balayssac, S. (2009). . J. Pharmaceut. Biomed, 50, 602-.
Ahn, S. (2009). . Rapid Commun. Mass Spectrom, 23, 3158-.
Liang, Q. L. (2006). . J. Pharmaceut. Biomed, 40, 305-.
Man, C. N. (2009). . J. Chromatogr. A, 1216, 8426-.
Gryniewicz, C. M. (2009). . J. Pharmaceut. Biomed, 49, 601-.
Jaesung Pyo. (2012). Determination of the PDE-5 Inhibitors and Their Analogues by GC-MS and TMS Derivatization. Mass Spectrometry Letters, 3(1), 15-17. http://dx.doi.org/10.5478/MSL.2012.3.1.015.
Bogusz, M. J. (2006). . J. Pharmaceut. Biomed, 41, 554-.
Zhang, Y. (2010). . J. Sep. Sci, 33, 2109-.
Gratz, S. R. (2006). . Rapid Commun. Mass Spectrom, 20, 2317-.
van der Heeft, E. (2009). . J. Am. Soc. Mass Spectrom, 20, 451-.
Holman, S. W. (2010). . J. Anal. Chem, 82, 2347-.