• P-ISSN2233-4203
  • E-ISSN2093-8950

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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Simultaneous Determination of Synthetic Phosphodiesterase-5 Inhibitors in Dietary Supplements by Liquid Chromatography-High Resolution/Mass Spectrometry

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2012, v.3 no.2, pp.50-53
https://doi.org/10.5478/MSL.2012.3.2.50
Kim So-Hee (Korea Institute of Science and Technolog)
Kim Ho Jun (Korea Institute of Science and Technology)
Son Junghyun (Korea Institute of Science and Technolog)
Jeon Byoung Wook (Korea Institute of Science and Technolog)
Jeong Eun Sook (Inje University)
Cha Eun Ju (Korea Institute of Science and Technolog)
Lee Jaeick (Korea Institute of Science and Technolog)
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Abstract

After success of sildenafil for the treatment of erectile dysfunction, a large number of its analogues have beenapproved from FDA. Recently, the illegal dietary supplements which include sildenafil, vardenafil, tadalafil, or analogues ofthese drugs as ingredient have been widely distributed. Therefore, the determination of the residue of synthetic phosphodiesterase-5 (PDE-5) inhibitors in dietary supplements is highly required due to indiscriminate and unintentional overdose causednausea, chest pains, fainting and irregular heartbeat. In this paper, we report a rapid and sensitive analytical method for thesimultaneous determination of nine phosphodiesterase-5 inhibitors by liquid chromatography-high resolution mass spectrometry. The present method was found to be accurate and reproducible with 40 μg/g of the limit of quantification for the nine PDE-5inhibitors. The developed method can be successfully applied to the analysis of the seven illegal dietary supplements.

keywords
LC-HR/MS LTQ-Orbitrap Phosphodiesterase-5 inhibitor Sildenafil


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Submission Date
2012-05-29
Revised Date
2012-06-19
Accepted Date
2012-06-19
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