ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

논문 상세

    An optimized cupric reducing antioxidant capacity (CUPRAC) method for assessment of xanthine oxidase inhibition activity

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2023, v.36 no.1, pp.44-52
    https://doi.org/10.5806/AST.2023.36.1.44
    Ahlam Majid Azeez (College of Science, University of Babylon, Iraq)
    Mahmoud Hussain Hadwan (College of Science, University of Babylon, Iraq)
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    Abstract

    This protocol clarifies a simple and precise method for measuring the activity of xanthine oxidase (XO) enzyme inhibitor. XO enzyme, which accelerates oxidative stress-related disorders through its capacity to generate hydrogen peroxide and superoxide anion radicals (O2 •−), has been found to be inhibited by several plant extracts. Enzyme samples were incubated with a suitable buffer containing adequate amounts of xanthine as a substrate to determine XO activity. The method depends on direct measurements of uric acid and hydrogen peroxide production to test XO with and without interference. The CUPRAC reagent (Cu(Nc)2 2+) was used to inhibit enzyme reaction after incubation was complete. The generated urate and peroxide reduced the Cu(II)- neocuproine complex (Cu(Nc)2 2+) to a brightly colored Cu(I)-neocuproine complex (Cu(Nc)2 +), which was assessed with a spectrophotometer at 450 nm. XO activity was found to be directly related to the increased absorbance of the colored Cu(I)-neocuproine complex (Cu(Nc)2 +). To eliminate catalase enzyme interference, the proposed method used sodium azide and was validated against XO activity using the UV method in matched samples with t-test analysis. The proposed assay can determine XO activity with high precision, as indicated by the correlation coefficient (R2 = 0.9935) from comparison with the reference protocol.

    keywords
    CUPRAC method, neocuproine complex, xanthine oxidase, catalase, polyphenolics, xanthine oxidase inhibitor


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