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- E-ISSN 2288-8985
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Determination of Histamine by Reverse-Phase High Performance Liquid Chromatography with Fluorescence Detection
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1996, v.9 no.2, pp.198-202
Ryu,
H., Kim,
H., Kim,
S., Kim,
D., Lee,
Y., Kim,
H., An,
N., &
Shin,
T.
(1996). Determination of Histamine by Reverse-Phase High Performance Liquid Chromatography with Fluorescence Detection. Analytical Science and Technology, 9(2), 198-202.
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Abstract
A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatization such as pH, reaction time and the concentration of FMOC were investigated. Linearity of calibration curve was obtained between <TEX>$0.1{\mu}g/ml$</TEX> and <TEX>$0.5{\mu}g/ml$</TEX>(r
- keywords
- HPLC analysis, Histamine, 9-Fluorenylmethyl chloroformate
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