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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

定志丸과 시스플라틴의 神經膠芽細胞腫에 對한 細胞枯死 機轉硏究

Mechanisms of Apoptosis by Combination with Jeongjihwan and Cisplatin in Human Glioblastoma Cells

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.2, pp.1-12
신학수 (원광대학교 한의학전문대학원)
이선우 (원광대학교 한의학전문대학원)
이문구 (원광대학교 한의학전문대학원)
윤종민 (원광대학교 한의학전문대학원)
이인 (원광대학교 한의과대학)
신선호 (원광대학교)
문병순 (원광대학교 한의학전문대학원)
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Abstract

Objectives : Malignant gliomas are often treated with cisplatin (cis-diaminedichloroplatinum (II), CDDP) and radiation but results remain unsatisfactory. Since malignant glioma displays moderate resistance to conventional therapy, a new treatment modality is needed to improve the outcome of patients with these tumors.The aim of this study was to investigate the effects of the combined use of Jeongjihwan(JJH) and cisplatin (CDDP) on cultured malignant glioma cells, A172.Method & Results : The combined use of cisplatin and Jeongjihwan had synergistic effects on A172 cells during 24 hr-incubation. This treatment resulted in decrease of cell viability, which was revealed as apoptosis characterized by activation of caspase-3 protease as well as cleavage of poly ADP-ribose polymerase (PARP) with change of mitochondrial membrane potential transition. The expression of members of the Bcl-2 protein family was modulated during co-treatment with Jeongjihwan and cisplatin. Activation of caspase-3 and mitochondrial alterations were central to co-treatment with Jeongjihwan and cisplatin-induced apoptosis.Conclusion : We conclude that co-treatment with Jeongjihwan and cisplatin-induced activation of the mitochondrial pathway enables cell death. Also, we suggest the combined theory of JJH and cisplatin could be a useful method for glioblastoma.

keywords
Jeongjihwan, Apoptosis, Human Glioblastoma Cells


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