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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effect of Chungpaesagan-tang on Ischemic Damage Induced by Middle Cerebral Artery Occlusion in Diabetic rats

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.2, pp.217-230







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Abstract

Objectives; Chungpaesagan-tang (CPSGT) which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. Researching the effect of CPSGT on focal cerebral ischemia in normal, diabetic rats and SHR, focal cerebral ischemia was induced by transient MCAO, and after onset CPSGT was administrated.Methods; Rats (Sprague-Dawley) were divided into four groups: Sham operated group, MCA occluded group, CPSGT administrated group after MCA occlusion, and Normal group. The MCA was occluded by intraluminal method. CPSGT was administrated orally twice (1 and 4 hours) after middle cerebral artery occlusion. All groups were sacrificed at 24 hours after the surgery. The brain tissue was stained with 2% triphenyl tetrazolium chloride (TTC) or 1% cresyl violet solution, to examine effect of CPSGT on ischemic brain tissue. The blood samples were obtained from the heart of rats. Tumor necrosis factor-α level and Interleukin 6 level of serum was measured from sera using Enzyme-Linked Immunoabsorbent Assay (ELISA). Then changes of immunohistochemical expression of TNF-α in ischemic damaged area were observed. Results; In NC+MCAO+CP and DM+MCAO+CP, CPSGT significantly (p<0.001) decreased the number of neuron cells compared to the each control group. CPSGT markedly reduced (P<0.01) the infarct size of the forebrain in distance from the interaural line on cerebral ischemia in diabetic rats. CPSGT significantly reduced the TNF-α expression in penumbra region of damaged hemisphere in diabetic rats. Conclusions; Thus, CPSGT had a protective effect on cerebral ischemia in SD rats. Especially, in diabetic rats compared with normal SD rat.

keywords
Cerebral ischemia, MCAO, Diabetes mellitus, TNF-α, IL-6, Chungpaesagan-tang, Cerebral ischemia, MCAO, Diabetes mellitus, TNF-α, IL-6, Chungpaesagan-tang


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