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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects of Sunghyangchungisan(SHCS) on oxidant-induced cell death in human neuroglioma cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.2, pp.63-76



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Abstract

Objective:Reactive oxygen species (ROS) have been implicated in the pathogenesis of a wide range of acute and long-term neurodegenerative diseases. This study was undertaken to examine whether Sunghyangchungisan (SHCS), a well-known prescription in Korean traditional medicine, might have beneficial effects on ROS-induced brain cell injury. Methods:Human neuroglioma cell line A172 and H2O2 were employed as an experimental model cell and oxidant. Results;SHCS effectively protected the cells against both the necrotic and apoptotic cell death induced by H2O2. The effect of SHCS was dose-dependent at concentrations ranging from 0.2 to 5 mg/㎖. SHCS significantly prevented depletion of cellular ATP and activation of poly (ADP-ribose) polymerase induced by H2O2. It also helped mitochondria to preserve its functional integrity estimated by MTT reduction ability. Furthermore, SHCS significantly prevented H2O2-induced release of cytochrome c into cytosol. Determination of intracellular ROS showed that SHCS might exert its role as a powerful scavenger of intracellular ROS. Conclusion:The present study provides clear evidence for the beneficial effect of SHCS on ROS-induced neuoglial cell injury. The action of SHCS as an ROS-scavenger might underlie the mechanism.

keywords
Sunghyangchungisan(SHCS), neuroglioma cells, Reactive oxygen species (ROS), Sunghyangchungisan(SHCS), neuroglioma cells, Reactive oxygen species (ROS)


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