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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

계지의 항염 효과에 관한 연구

The Anti-inflammatory Effect of Cinnamomi Ramulus

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.2, pp.140-151
박히준 (경희대학교)
이지숙 (경희대학교)
이재동 (경희대학교)
김남재 (경희대학교)
표지희 (경희대학교)
강전모 (경희대학교)
최일환 (경희대학교)
김수영 (경희대학교)
심범상 (경희대학교)
이제현 (경희대학교)
임사비나 (경희대학교)
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Abstract

Objective : Cinnamomi Ramulus (CR) that the young twig of Cinnamomum loureirii NEES has been used for treating symptoms related to pain, rheumatic arthritis and inflammation in Korean herb medicine. This study was carried out to investigate the anti-inflammatory effect of CR in vivo and in vitro. Methods : Extracts of CR were prepared and the chemical components of the extracts were examined by gas chromatography-mass spectrometry (GC-MS). The extracts were administrated to the rat paw edema model induced by carrageenan to evaluate the anti-inflammatory effect of CR. The expressions of nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 were also quantified in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages to survey the effect of CR in vitro. The main components were cinnamaldehyde and coumarin. Results : We examined the anti-inflammatory activity of the 80% ethanol extract of Cinnamomi Ramulus in vivo by using carrageenan-induced rat paw edema model. Maximum inhibition of 54.91% was noted at the dose of 1000 ㎎/㎏ after 2 hours of drug administration in carrageenan-induced rat paw edema and this showed a potent anti-inflammatory effect. Conclusion : The results showed that Cinnamomi Ramulus suppressed dose-dependently LPS-induced NO production in RAW 264.7 macrophages and also decreased iNOS protein expression. Cinnamomi Ramulus also showed a significant inhibitory effect in LPS-induced PGE2 production and COX-2 expression.

keywords
Cinnamomi Ramulus, anti-inflammatory effect, carrageenan, rat paw edema, nitric oxide, cyclooxygenase-2


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