- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
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Amygdalin Extract from Armeniacae semen Induces Apoptosis in Human COLO 201 Colon Cancer Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2005, v.26 no.4, pp.108-121
(College of Oriental Medicine, Kyungwon University)
(Kyung-Won University)
(Kyung-Won University)
Kyung-Nam,
K.
, Yun-kyung,
S.
, &
Hyung-Ho,
L.
(2005). Amygdalin Extract from Armeniacae semen Induces Apoptosis in Human COLO 201 Colon Cancer Cells. Journal of Korean Medicine, 26(4), 108-121.
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Abstract
Backgrounds : Amygdalin (D-mandelonitrile B-gentiobioside), a cynogenic compound, is found in sweet and bitter almond, Persicae semen, and Armeniacae semen. Aqueous extract of amygdalin was made from Armeniacae semen and used in this study. Objectives : Apoptosis is a very important mechanism in cancer treatment. In the present study, it was investigated whether amygdalin induces apoptotic cell death in human COLO 201 colon cancer cells. Materials and Methods : For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6- diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, reverse transcription-polymerase chain reaction(PR-PCR), western blot analysis, and caspase-3 enzyme assay were performed on COLO 201 cells. Cells treated with amygdalin exhibited several characteristics of apoptosis. Results : Amygdalin treatment enhanced Bax expression and suppressed Bcl-2 expression in COLO 201 cells. Amygdalin also was shown to increase the caspase-3 activity. Conclusions : Amygdalin induces apoptotic cell death via Bax-dependent caspase-3 activation in COLO 201 cells.
- keywords
- Amygdalin, human COLO 201 colon cancer cells, apoptosis, caspase-3
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