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Determination of Homocysteine, Methionine, Cysteine in Human Plasma with SPME/GC-MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2000, v.13 no.6, pp.736-742
Yoo,
E., Kim,
J., &
Myung,
S.
(2000). Determination of Homocysteine, Methionine, Cysteine in Human Plasma with SPME/GC-MS. Analytical Science and Technology, 13(6), 736-742.
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Abstract
The purpose of this study was to determine the homocysteine (Hey), methionine (Met) and cysteine (Cys) using solid phase micro-extraction (SPME)/gas chromatography (GC)-mass spectrometry (MS) in human plasma and to correlate between the plasma concentration of homocysteine with coronary artery disease. The homocysteine, methionine and cysteine in blood can be used as biomarkers for the risk assessment of vascular disease. The plasma homocysteine level for the coronary artery disease patients was higher than general patients. The concentration ranges of the Hcy, Met and Cys for coronary artery disease patients were <TEX>$18.47-33.38{\mu}mol/L$</TEX>, <TEX>$30.16-55.72{\mu}mol/L$</TEX> and <TEX>$183.16-387.32{\mu}mol/L$</TEX>, respectively. This method showed good sensitivity and convenience.
- keywords
- homocysteine, methionine, cysteine, SPME, GC-MS
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