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- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Animal species identification by co-amplification of hypervariableregion 1 (HV1) and cytochrome b in mitochondrial DNA
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2005, v.18 no.3, pp.257-262
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(2005). Animal species identification by co-amplification of hypervariableregion 1 (HV1) and cytochrome b in mitochondrial DNA. Analytical Science and Technology, 18(3), 257-262.
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Abstract
Mitochondrial DNA (mt DNA) sequence analysis has been a useful tool for species identification of animals and human individuals. Two hypervariable regions (HV1 and HV2) in control region of mitochondrial genome were analyzed for human individual identification. In case of animal species identification, several genes on mt DNA such as cytochrome b (cytb), RNAs, cytochrome oxidases (CO) were used. In this study, co-amplification of HV1 and cytb was carried out in order to check the contamination of animal DNA and to verify the human DNA. The primer sets used in PCR were H15997/L16236 for HV1 and H14724/L15149 for cytb. PCR products for HV1 and cytb were 239bp and 425bp, respectively. The appearance of two bands on agarose gel implied the DNA came from human, however the single band of cytb gene represented the non-human animal DNA.
- keywords
- Co-amplification, mitochondrial DNA, cytochrome b, HV1, species identification
Reference
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(1998) Speceis-identification using genetic tools: the value of nuclear and mitochondrial gene sequences in whale conservation,
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(1999) Multiplex amplification of mitochondrial DNA for human and species identification in forensic evaluation,
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(1989) Dynamics of mitochondrial DNA evolution in animals,
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(2003) Species identification of rhinoceros horns using the cytochrome b gene,
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