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  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

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    Characterization of biotin-avidin recognition system constructed on the solid substrate

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2005, v.18 no.6, pp.460-468
    임정혁 (청주대학교)
    임정혁. (2005). . 분석과학, 18(6), 460-468.
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    Abstract

    The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. <TEX>$60{\AA}$</TEX> thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and <TEX>$7.5{\times}10^{-12}mol/cm^2$</TEX>, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

    keywords
    Biotin, avidin, stepwise self-assembly, SERRS, AFM, SPR


    참고문헌

    1

    (1991) An Introduction to Ultrathin Organic Filmsfrom Langmuir-Blodgett to Self-assembly, AcademicPress

    2

    (1971) Phys. 241,

    3

    (1991) Optical Properties of Thin Solid Films,

    4

    (1959) Physik und Chemie der Grenzflachen,

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