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- E-ISSN 2288-8985
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Quantification of seleno proteins in Korean blood serum using solid phase extraction and affinity chromatography-inductively coupled plasma/mass spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2014, v.27 no.2, pp.92-99
https://doi.org/10.5806/AST.2014.27.2.92
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(2014). Quantification of seleno proteins in Korean blood serum using solid phase extraction and affinity chromatography-inductively coupled plasma/mass spectrometry. Analytical Science and Technology, 27(2), 92-99, https://doi.org/10.5806/AST.2014.27.2.92
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Abstract
Interferences were removed using anion exchange solid phase extraction (AE SPE) in quantificationof selenoproteins in Korean human blood serum with affinity high performance liquid chromatography (AFHPLC)-inductively coupled plasma/mass spectrometry (ICP/MS). The average selenium level obtained for healthyKoreans was 94.3 ± 2.3 ng g−1 using isotope dilution method. AE SPE was coupled to AF column to separate3 selenoproteins, glutathione peroxidase GPx, selenoprotein SelP, and selenoalbumin SeAlb. Post column isotopedilution was employed to quantify the proteins. The certified reference material of human blood serum BCR-637 was analyzed to give total selenoprotein concentration of 85.4 ± 3.4 ng g−1, which agreed well with thereference value of 81 ± 7 ng g−1. The pooled concentration of GPx, SelP, and SeAlb from healthy Koreans(n=20) was 12.1 ± 1.4 ng g−1, 57.2 ± 2.0 ng g−1, and 20.0 ± 1.9 ng g−1, respectively. The sum of selenoproteinsis 89.3 ng g−1, which is about the same as the total selenium concentration of 94.3 ng g−1. The fact suggeststhat selenium in blood serum is mostly consisted of selenoproteins. After the removal of interference, GPxshowed a significant decrease (more than 50%) from 25.0 ng g−1 to 12.1 ng g−1. It was identified that theinterference in blood serum was mostly from GPx and the use of AE SPE was proven to be efficient ineliminating Cl and Br that cause interference to GPx.
- keywords
- Korean blood serum, AF HPLC-ICP/MS, selenoproteins, GPx, SelP, SeAlb, solid phase extraction
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