- P-ISSN 1225-0163
- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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LC-QTOF-MS를 이용한 발기부전치료제 유사물질의 fragmentation pathway 분석
Identification and evaluation of fragmentation pathways of PDE-5 inhibitor analogues using LC-QTOF-MS
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.4, pp.278-287
https://doi.org/10.5806/AST.2015.28.4.278
(식품의약품안전처)
(식품의약품안전처, 식품의약품안전평가원)
(식품의약품안전처 식품의약품안전평가원 독성평가연구부 첨단분석팀)
(식품의약품안전처)
(식품의약품안전처)
(식품의약품안전청)
(식품의약품안전처)
(식품의약품안전처, 식품의약품안전평가원)
(식품의약품안전처 식품의약품안전평가원 독성평가연구부 첨단분석팀)
(식품의약품안전처)
(식품의약품안전처)
(식품의약품안전청)
(식품의약품안전처)
도정아,
노은영,
윤순병,
박형준,
조수열,
박성관,
&
윤창용.
(2015). LC-QTOF-MS를 이용한 발기부전치료제 유사물질의 fragmentation pathway 분석. 분석과학, 28(4), 278-287, https://doi.org/10.5806/AST.2015.28.4.278
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Abstract
Phosphodiesterase type 5 inhibitors (PDE-5 inhibitors) are used in the treatment of erectile dysfunction. In recent years, a number of reports have been conducted on dietary supplements contaminated with PDE-5 analogues. In this study, 58 analogues of PDE-5 inhibitors were sorted into five groups: tadalafil, sildenafil, hongdenafil, vardenafil, and other analogues. These analogues were then evaluated using a liquid chromatographyquadrupole- time of flight mass spectrometry (LC-QTOF-MS) electrospray ionization mass method. Each compound has a unique fragmentation ion, which can be easily analyzed qualitatively. The fragmentation pathways of the analogues were elucidated based on the QTOF-MS and MS/MS data. Common ions were confirmed for each group by analyzing the structural characteristics and fragmentation pathways. Specifically, common ions were observed at m/z 169.08 and 135.04 (tadalafil analogues), m/z 311.15 and 283.12 (sildenafil analogues and hongdenafil analogues), and m/z 312.16 and 151.09 (vardenafil analogues). The advantage of this method is that the structure of unknown components can be determined by interpreting the product ions. Hence, the developed method can be used for the identification of unknown compounds. Fragmentation pathways may also aid in the detection and identification of PDE-5 inhibitor analogues.
- keywords
- PDE-5, LC-QTOF-MS, fragmentation pathway, identification, unknown compounds
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