- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Apply for Authority
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Inhibitory effect of Angelica gigas extract powder on induced inflammatory cytokines in rats osteoarthritis
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2015, v.28 no.4, pp.260-269
https://doi.org/10.5806/AST.2015.28.4.260
&
(2015). Inhibitory effect of Angelica gigas extract powder on induced inflammatory cytokines in rats osteoarthritis. Analytical Science and Technology, 28(4), 260-269, https://doi.org/10.5806/AST.2015.28.4.260
- Downloaded
- Viewed
Abstract
The protective effects of extract powder of Angelica gigas on the degeneration of the articular cartilage in rats was investigated with monosodium iodoacetate (MIA)-induced osteoarthritis, The treatment of high concentration (50 μg/mL) of Angelica gigas effectively inhibited nitric oxide (NO) production induced by interleukin-1α (IL-1α) without any cytotoxicity. Specifically, mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dose dependently reduced by extract powder of Angelica gigas. Importantly, mRNA expression in articular cartilage of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were clearly reduced. The inflammatory cytokines in blood were also reduced as well. These results suggested that the protective effects on the degeneration of the articular cartilage was derived from the inhibitory effects of mRNA and protein expression of tested inflammatory cytokines which is linked to prevent the degradation of proteoglycan (PG), the main matrix content in articular cartilage. Meanwhile, the 2 hrs incubation of decursin, a major compound of extract powder in rat whole blood rapidely converted decursin into decursinol which shows string anti-inflammatory activity. The coverted decursinol was detected after 8 hrs in whole blood by LC-MS/MS. Conclusively, the inhibitory effects of inflammatory cytokines production in osteoarthritis may be derived from the production of decursinol, which performs against inflammatroy cytokines like TNF-α, IL-1β‚ and IL-6.
- keywords
- Angelica gigas, osteoarthritis, inflammatory cytokines, <TEX>$TNF-\alpha$</TEX>, decursin, decursinol
Reference
1
1. R. Mardones, C. M. Jofré and J. J. Minguell, Int. J. Stem. Cells., 8(1), 48-53 (2015).
2
2. D. Pereira, E. Ramos and J. Branco, Acta Med. Port., 28(1), 99-106 (2015).
3
3. M. Schuliga, Biomolecules, 5(3), 1266-1283 (2015).
4
4. M. L. Buckley and D. P. Ramji, Biochim Biophys Acta, 1852(7), 1498-1510 (2015).
5
5. B. Song, G. Huang, Y. Xiong, J. Liu, L. Xu, Z. Wang, G. Li, J. Lu and S. Guan, J. Med. Food., 16(11), 997- 1003 (2013).
6
6. M. Endale, S.C. Park, S. Kim, S.H. Kim, Y. Yang, J.Y. Cho and M.H. Rhee, Immunobiology, 218(12), 1452-1467 (2013).
7
7. C. Y. Wenham, M. McDermott and P.G. Conaghan, Curr Pharm Des., 21(17), 2206-2215 (2015).
8
8. O. Ham, C. Y. Lee, R. Kim, J. Lee, S. Oh, M. Y. Lee, J. Kim, K. C. Hwang, L. S. Maeng and W. Chang, Int. J. Mol Sci., 16(7), 14961-14978 (2015).
9
9. T. Leroux, J. Chahal, D. Wasserstein, N. N. Verma, A. A. Romeo. Sports Health., 7(4), 303-307 (2015).
10
10. T. Manyanga, M. Froese, R. Zarychanski, A. Abou-Setta, C. Friesen, M. Tennenhouse, B. L. Shay, BMC Complement Altern Med., 14, 312 (2014).
11
11. N. Schuelert and J. J. McDougall, Neurosci. Lett., 465(2), 184-188 (2009).
12
12. O. V. Nemirovskiy, M. R. Radabaugh, P. Aggarwal, C. L. Funckes-Shippy, S. J. Mnich, D. M. Meyer, T. Sunyer, W. Rodney Mathews and T. P. Misko. Nitric Oxide., 20(3), 150-156 (2009).
13
13. S. Y. Jeong, H. M. Kim, K. H. Lee, K. Y. Kim, D. S. Huang, J. H. Kim and R. S. Seong, Chem. Pharm. Bull. (Tokyo)., 63(7), 504-511 (2015).
14
14. K. Tomiyama, A. Yamaguchi, T. Kuriyama and Y. Arakawa, J. Immunotoxicol, 6(3), 184-193 (2009).
15
15. Y. Huang, Y. Lu, L. Zhang, J. Yan, J. Jiang and H. Jiang, Int. J. Mol. Sci., 15(3), 4049-4059 (2014).
16
16. M. Kim, Y. G. Park, H. J. Lee, S. J. Lim and C. W. Nho, J. Agric. Food Chem., 63(22), 5428-5438 (2015).
17
17. Y. Shen, S. Yang, Z. Shi, T. Lin, H. Zhu, F. Bi, A. Liu, X. Ying, H. Liu, K. Yu and S. Yan, Inflammation, 38(2),736-744 (2015).
18
18. J. H. Kwon, M. S. Han, B. M. Lee and Y. M. Lee, Anal. Sci. Tech., 28(1), 72-77 (2015).
19
19. J. S. Song, J. W. Chae, K. R. Lee, B. H. Lee, E. J. Choi, S. H. Ahn, K. I. Kwon and M. A. Bae, Xenobiotica, 41(10), 895-902 (2011).
Other articles from this issue
- Spectrofluorimetric determination of trace Co(II) in aqueous samples with quinalizarin-β-cyclodextrin inclusion complex
- Inhibitory effect of Angelica gigas extract powder on induced inflammatory cytokines in rats osteoarthritis
- QSPR model for the boiling point of diverse organic compounds with applicability domain
- Identification and evaluation of fragmentation pathways of PDE-5 inhibitor analogues using LC-QTOF-MS
- A study on the distribution of glitter particles from an university building
- Determination of streptomycin in kiwifruit samples using LC-ESI-MS/MS
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.