- P-ISSN 1225-0163
- E-ISSN 2288-8985
- 권한신청
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
논문 상세
Home > 논문 상세
Identification of triacylglycerols in coix seed extract by preparative thin layer chromatography and liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2017, v.30 no.2, pp.102-111
https://doi.org/10.5806/AST.2017.30.2.102
(경희대학교)
(경희대학교)
(경희대학교)
(경희대학교)
(경희대학교)
(경기대학교)
(경희대학교)
(경희대학교)
(경희대학교)
(경희대학교)
(경기대학교)
심희정,
홍종기,
이슬기,
박나현,
김윤아,
&
조현우.
(2017). . 분석과학, 30(2), 102-111, https://doi.org/10.5806/AST.2017.30.2.102
- 다운로드 수
- 조회수
Abstract
Here we reported a methodology for identification of triacylglycerols (TAGs) and diacylglycerols (DAGs) in coix seed by preparative thin layer chromatography (prep-TLC) and non-aqueous reversed-phase liquid chromatography (NARP LC)-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (MS/MS). Lipid components were extracted from coix seed by reflux extraction using n-hexane for 3 hr. TAGs and DAGs in coix seed extract were effectively purified and isolated from matrix interferences by prep-TLC and then analyzed by LC-APCI-MS and MS/MS for identification. TAGs were effectively identified taking into consideration of their LC retention behavior, APCI-MS spectra patterns, and MS/MS spectra of [DAG]+ ions. In MS/MS spectra of TAGs, diacylglycerol-like fragment [DAG]+ ions were useful to identify TAGs with isobaric fragment ions. Based on an established method, 27 TAGs and 8 DAGs were identified in coix seed extract. Among them, 15 TAGs and 8 DAGs were for the first time observed in coix seed. Interestingly, some of TAGs isolated by prep-TLC were partly converted into DAGs through probably photolysis process during storing in room temperature. Thus, degradation phenomenon of TAGs should be considered in the quality evaluation and nutritional property of coix seed. LC-APCI-MS/MS combined with prep-TLC will be practical method for precise TAG and DAG analysis of other herbal plants.
- keywords
- triacylglycerol, diacylglycerol, coix seed, Prep-TLC, LC-APCI-MS/MS
참고문헌
1
1. C. C. Kuo, , W. Chiang, G. P. Liu, Y. L. Chien, J. Y. Chang, C. K. Lee, J. M. Lo, S. L. Huang, M. C. Shih, and Y. H. Kuo, J. Agric. Food Chem., 50, 5850-5855 (2002).
2
2. D. W. Huang, Y. H. Kuo, F. Y. Lin, Y. L. Lin, and W. Chiang, J. Agric. Food Chem., 57, 2259-2266 (2009).
3
3. Tanimura, Chem. Pharm. Bull., 9, 47-53 (1961).
4
4. S. O. Kim, S. J. Yun, B. Jung, E. H. Lee, D. H. Hahm, I. Shim, and H. J. Lee, Life Sci., 75, 1391-1404 (2004).
5
5. F. Yu, J. Gao, Y. Zeng, and C. X. Liu, J. Ethnopharmacol., 119, 252-258 (2008).
6
6. M. J. Jiménez, L. Esteban, A. Robles, E. Hita, P. A. González, M. M. Muñío, and E. Molina, Process Biochem., 45, 407-414 (2010).
7
7. M. C. Michalski, C. Genot, C. Gayet, C. Lopez, F. Fine, F. Joffre, J. L. Vendeuvre, J. Bouvier, J. M. Chardigny, and K. Raynal-Ljutovac, Prog. Lipid Res., 52, 354-373 (2013).
8
8. S. D. Stamatov and J. Stawinski, Org. Biomol. Chem., 5, 3387-3800 (2007).
9
9. Z. M. Xiang, M. ZHU, B. L. Chen, and Y. Chen, Chin. J. Chinese materia medica, 30, 1436-1438 (2005).
10
10. Fan Zhu, Trends Food Sci. Tech., 61, 160-175 (2017).
11
11. M. H. Lee, H. K. Park, and I. W. Kim, Anal. Sci. Tech., 19, 189-193 (2006).
12
12. M. Lisa, M. Holcapek, T. Rezanka, and N. Kabatova, J. Chromatogr. A., 1146, 67-77 (2007).
13
13. C. Ruiz-Samblás, L. Cuadros-Rodríguez, A. González-Casado, F. P. Rodríguez García, D. L. Mata-Espinosa, P. and J. M. Bosque-Sendra, Anal. Bioanal. Chem., 399, 2093-2103 (2011).
14
14. E. D. Dodds, M. R. McCoy, L. D. Rea, and J. M. Kennish, Lipids 40, 419-428 (2005).
15
15. H. J. Sim, J. H. Kim, S. K. Lee, E. H. Kim, Y. H. Jin, E. K. Seo, and J. Hong, Bull. Korean Chem. Soc., 36, 1707-1709 (2015).
16
16. M. Fasciotto and A. D. Pereira Netto, Talanta, 81, 1116-1125 (2010).
17
17. Q. Zhou, B. Gao, X. Zhang, Y. Xu, H. Shi, and L. Yu, Food Chem., 143, 199-204 (2014).
18
18. A. Jakab, K. Héberger, and E. Forgács, J. Chromatogr. A, 976, 255-263 (2002).
19
19. A. Acheampong, N. Leveque, A. Tchapla, and S. Herson, J. Chromatogr. A, 1218, 5087-5100 (2011).
20
20. M. Lísa and M. Holcãpek, Anal. Chem., 85, 1852-1859(2013).
21
21. T. Rezanka, L. Nedbalová, and K. Sigler, J. Chromatogr. A, 1467, 261-269 (2016).
22
22. H. C. Lu, P. L. Jiang, Light R. C. Hsu, C. L. Chyan, and Jason T. C. Tzen, Biosci. Biotechnol. Biochem., 74, 1841-1847 (2010).
권호 내 다른 논문
- Ion chromatographic determination of chlorite and chlorate in chlorinated food using a hydroxide eluent
- Determination of volatile compounds by headspace-solid phase microextraction – gas chromatography / mass spectrometry: Quality evaluation of Fuji apple
- Development of dry-origin latent footwear impression on non-porous and semi-porous surfaces using a 5-methylthioninhydrin and L-alanine complex
- Development of simple HPLC-UV method for discrimination of Adenophorae Radix
- Thermodynamics of the binding of Substance P to lipid membranes
- Effect of centrifugation on tryptic protein digestion
- Identification of triacylglycerols in coix seed extract by preparative thin layer chromatography and liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.