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- E-ISSN 2288-8985
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Thermodynamics of the binding of Substance P to lipid membranes
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2017, v.30 no.2, pp.89-95
https://doi.org/10.5806/AST.2017.30.2.89
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(2017). Thermodynamics of the binding of Substance P to lipid membranes. Analytical Science and Technology, 30(2), 89-95, https://doi.org/10.5806/AST.2017.30.2.89
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Abstract
The thermodynamic functions for the binding of the peptide Substance P (SP) on the surface of lipid vesicles made of various types of lipids were obtained by using isothermal titration calorimetry. The reaction enthalpies measured from the experiments were -0.11 to -4.5 kcal mol−1. The sizes of the lipid vesicles were measured with dynamic light scattering instrument in order to get the correlation between the reaction enthalpies and the vesicle sizes. The bindings of SP on the lipid vesicles with diameter of 37 to 108 nm were classified into the enthalpy-driven reaction or the entropy-driven reaction according to the size of the lipid vesicles. For the enthalpy-driven binding reaction, the significance of the electrostatic interactions between SP and lipid molecules was affirmed from the experimental results of the DMPC/DMPG/DMPH and DMPC/DMPS/DMPH vesicles as well as the importance of the hydrophobic interactions between hydrophobic groups of SP and lipid molecules.
- keywords
- thermodynamics, partitioning, Substance P, lipid vesicle
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