ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

논문 상세

Home > 논문 상세
  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Development of simple HPLC-UV method for discrimination of Adenophorae Radix

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2017, v.30 no.2, pp.82-88
https://doi.org/10.5806/AST.2017.30.2.82
Thi Phuong Duyen Vu (충남대학교)
강종성 (충남대학교)
김경태 (충남대학교)
Yen Pham (University of Science and Technology of)
Hai Ying Bao (Jilin Agricultural University)
  • 다운로드 수
  • 조회수

Abstract

Adenophorae Radix (AR) is a frequently used medicinal herb; because of its popularity, products containing similar herbal products are often sold as substitutes, especially if their morphology is similar. However, any analytical method to identify AR based on quantitative analysis is not registered in Korea, Japan and China Pharmacopoeias. This study developed a simple HPLC method to discriminate between authentic AR and substitutes. Linoleic acid was used as a marker compound of AR. Our optimized HPLC-UV conditions included a mobile phase of 90 % acetonitrile under isocratic condition, and a flow rate of 1.0 mL/min at room temperature. Detection wavelength was set at 205 nm. Linoleic acid was detected at 13.5 minutes for a total analysis time of 20 minutes. The standard herb of AR contained 0.025 % of linoleic acid, while four authentic AR samples and eight substitutes contained 0.040~0.071 % and 0.004~0.014 %, respectively. Comparison of the linoleic acid concentrations of the sample types to reference AR showed that among 12 samples, only the four samples were authentic. Thus, our HPLC-UV method, along with our suggested content criterion for linoleic acid concentration, can be used for the quick and accurate determination whether the herbal products are authentic AR or substitute.

keywords
Adenophorae Radix, HPLC-UV, linoleic acid, discriminant analysis


참고문헌

1

1. L. Packer, C. N. Ong, and B. Halliwell, Herbal and Traditional Medicine, 2nd Ed., CRC Press, United States, 2004.

2

2. The Ministry of Food and Drug Safety (MFDS), Adenophorae Radix, The Korean Herbal Pharmacopoeia, 4, 190 (2015).

3

3. China Food and Drug Administration, Chinese Pharmacopoeia commission (CP), 244-245 (2015).

4

4. D. Hong, T. Lammers and L. Klein, Adenophora genus, Flora of China, 19, 505-536 (2011).

5

5. K. C. Huang, The Pharmacology of Chinese herbs, 2nd Ed., CRC press, United States, 1998.

6

6. S. S. Roh, S. H. Kim, Y. C. Lee, and Y. B. Seo, Mediators Inflamm., 2008, 1-11 (2008).

7

7. S. H. Min, H. S. Han, and Y. J. Lee, Kor. J. Haerbol., 24(3), 109-119 (2009).

8

8. S. G. Li, H. Y. Chen, C. S. Yang, X. X. Wang, Z. J. Yang, Y. Tong, and W. Cho, Plos One, 8(2), e57604 (2013).

9

9. W. H. Woo and Y. J. Mun, Composition containing extracts from Adenophorae Radix for reducing the melanogenesis, Pat. KR100555242B1, Republic Korean Kongkae Taeho Kongbo, South Korea (2005).

10

10. R. Watson and V. Preedy, Botanical Medicine in Clinical Practice, 1st Ed., CABI, United Kingdom, 2008.

11

11. C. H. Hempen and T. Fischer, A materia medica for Chinese medicine: plants, minerals and animal products, 1st Ed., Elsevier Health Sciences, Germany, 2009.

12

12. K. J. Zhao, T. T. Dong, X. M. Cui, P. F. Tu, and K. W. Tsim, Am. J. Chin. Med., 31(06), 919-926 (2003).

13

13. S. H. Zheng, D. W. Liu, W. G. Ren, J. Fu, L. Huang, and S. Chen, Evid. Based Complement. Alternat. Med., 1-11 (2014).

14

14. Y. K. Park, H. H. Yoo, S. H. Baek, M. K. Park, S. H. Lee, C. M. Kim, and K. S. Lee, Kor. J. Pharmacogn., 34(1), 10-13 (2003).

15

15. K. I. Won, B. T. Zhao, J. H. Lee, D. U. Lee, Y. S. Kim, B. S. Min, J. K. Son, and M. H. Woo, Nat. Prod. Sci., 20(4), 243-250 (2014).

16

16. Y. P. Yoon, H. J. Lee, J. H. Hong, C. J. Lee, D. U. Lee, and S. K. Lee, Tuberc. Respir. Dis., 78(3), 210-217 (2015).

17

17. P. Jiang and Z. Gao, China. J. Mater. Med., 15(8), 486-512 (1990).

18

18. C. Konno, T. Saito, Y. Oshima, H. Hikino, and C. Kabuto, Planta Med., 42(3), 268-274 (1981).

19

19. H. X. Kuang, C. J. Shao, R. Kasai, K. Ohtani, Z. K. Tian, J. D. Xu, and O. Tanaka, Chem. Pharm. Bull., 39(9), 2440-2442 (1991).

20

20. J. H. Ward, J. Am. Stat. Assoc., 58, 236-244 (1963).

21

21. Ministry of Food and Drug Safety of Korea, Validation guideline for set-up of components profiling in herbal medicine, 2010.

22

22. National Center for Biotechnology Information, PubChem Compound Database, https://pubchem.ncbi.nlm.nih.gov /compound/702.

상단으로 이동

분석과학