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- E-ISSN 2288-8985
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- E-ISSN2288-8985
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Effect of novel luminol-based blood detection reagents on DNA stability
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2018, v.31 no.2, pp.71-77
https://doi.org/10.5806/AST.2018.31.2.71
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(2018). Effect of novel luminol-based blood detection reagents on DNA stability. Analytical Science and Technology, 31(2), 71-77, https://doi.org/10.5806/AST.2018.31.2.71
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Abstract
Detection of bloodstains is a very important process in scientific investigations, and luminol is often used for the detection of bloodstains that are not visible. Recently, new preparation methods of blood detection reagents based on luminol (BloodFlareA, B) were developed and reported to have higher active persistence and to be more economical than conventional blood detection reagent, BlueStar forensic. In this paper, we tested the specificity and effect of the BloodFlares (A and B) on DNA and compared them with those of BlueStar forensic. False positive results for the BloodFlares were not observed in semen, saliva, vaginal fluids, urine, sweat, and nasal discharge, but were observed in CuSO4, FeSO4, and bleach solutions, and the observed patterns were similar to those of BlueStar forensic. The effect on DNA was determined by analyzing the DNA yield, degradation index, and DNA profiling. Based on these results, we concluded that the BloodFlares based on luminol do not affect DNA stability and are applicable in forensics.
- keywords
- luminol, new preparation methods of blood detection reagents, BloodFlares, BlueStar forensic, specificity, effect on DNA
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