- P-ISSN 1225-0163
- E-ISSN 2288-8985
- 권한신청
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
논문 상세
Home > 논문 상세
Determination of methamphetamine and amphetamine enantiomers in human urine by chiral stationary phase liquid chromatography-tandem mass spectrometry
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2019, v.32 no.5, pp.163-172
https://doi.org/10.5806/AST.2019.32.5.163
(Forensic Genetics & Chemistry Division, Supreme Prosecutors' Office)
(대검찰청)
(Forensic Genetics & Chemistry Division, Supreme Prosecutors' Office)
(대검찰청)
(Forensic Genetics & Chemistry Division, Supreme Prosecutors' Office)
Yeong,
E.
S.
,
고범준,
&
Jin,
Y.
K.
(2019). . 분석과학, 32(5), 163-172, https://doi.org/10.5806/AST.2019.32.5.163
- 다운로드 수
- 조회수
Abstract
Methamphetamine (MA) is currently the most abused illicit drug in Korea and its major metabolite is amphetamine (AP). As MA exist as two enantiomers with the different pharmacological properties, it is necessary to determine their respective amounts in a sample. Thus a chiral stationary phase liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for identification and quantification of d-MA, l-MA, d-AP, and l-AP in human urine. Urine sample (200 μL) was diluted with pure water and purified using solid-phase extraction (SPE) cartridge. A 5-μL aliquot of SPE treated sample solution was injected into LC-MS/MS system. Chiral separation was carried out on the Astec Chirobiotic V2 column with an isocratic elution for each enantiomer. Identification and quantification of enantiomeric MA and AP was performed using multiple reaction monitoring (MRM) detection mode. Linear regression with a 1/x2 as the weighting factor was applied to generate a calibration curve. The linear ranges were 25-1000 ng/mL for all compounds. The intra- and inter-day precisions were within 3.6 %, while the intra- and inter-day accuracies ranged from -5.4 % to 11.8 %. The limits of detection were 2.5 ng/mL (d-MA), 3.5 ng/mL (l-MA), 7.5 ng/ mL (d-AP), and 7.5 ng/mL (l-AP). Method validation parameters such as selectivity, matrix effect, and stability were evaluated and met acceptance criteria. The applicability of the method was tested by the analysis of genuine forensic urine samples from drug abusers. d-MA is the most common compound found in urine and mainly used by abusers.
- keywords
- chiral separation, LC-MS/MS, d, l-methamphetamine, d, l-amphetamine, urine
참고문헌
1
1. Supreme Prosecutors' Office, White Paper on Drug-Related Crimes 2018, Seoul, Korea, 2019.
2
2. A. W. Brzeczko, R. Leech and J. G. Stark, Am. J. Drug Alcohol Abuse, 39, 284-290 (2013).
3
3. K. M. Andrews, J. Forensic Sci., 40, 551-560 (1995).
4
4. C. Chulathida and C. Summon, Curr. Opin. Psychiatry, 28, 269-274 (2015).
5
5. A. Gamma, R. Schleifer, W. Weinmann, A. Buadze and M. Liebrenz, PLoS One 11, e0166566 (2016).
6
6. United Nations Office on Drugs and Crime, World Drug Report 2019, Vienna, United Nations, 2019.
7
7. L. Li, T. Everhart, P. Jacob 3rd, R. Jones and J. Mendelson, Br. J. Clin. Pharmacol., 69, 187-192 (2010).
8
8. R. A. Glennon, Pharmacol. Biochem. Behav., 64, 251-256 (1999).
9
9. J. E. Mendelson, N. Uemura, D. S. Harris, R. P. Nath, E. Fernandez, P. Jacob 3rd, T. Everhart and R. T. Jones, Clin. Pharmacol. Ther., 80, 403-420 (2006).
10
10. J. E. Mendelson, D. McGlothlin, D. S. Harris, E. Foster, T. Everhart, P. Jacob 3rd and R. T. Jones, BMC Clin. Pharmacol., 8:4, 1-9 (2008).
11
11. R. W. Romberg, S. B. Needleman, J. J. Snyder and A. Greedan, J. Forensic. Sci., 40, 1100-1102 (1995).
12
12. W. A. W. Raihana, S. H. Gan and S. C. Tan, J. Chromatogr. B, 879, 8-16 (2011).
13
13. S. M. Wang, T. C. Wang and Y. S. Giang, J. Chromatogr. B, 816, 131-143 (2005).
14
14. J. P. Pascali, F. Bortolotti and F. Tagliaro, Electrophoresis, 33, 260-268 (2012).
15
15. E. M. Kim, H. S. Chung, K. J. Lee and H. J. Kim, J. Anal. Toxicol., 24, 238-244 (2000).
16
16. B. S. Foster, D. D. Gilbert, A. Hutchaleelaha and M. Mayersohn, J. Anal. Toxicol., 22, 265-269 (1998).
17
17. A. C. Lua, Y. Sutono and T. Y. Chou, Anal. Chim. Acta., 576, 50-54 (2006).
18
18. L. F. Ward, J. R. Enders, D. S. Bell, H. M. Cramer, F. N. Wallace and G. L. McIntire, J. Anal. Toxicol., 40, 255-263(2016).
19
19. E. R. Perez, J. A. Knapp, C. K. Horn, S. L. Stillman, J. E. Evans and D. P. Arfsten, J. Anal. Toxicol., 40, 201-207(2016).
20
20. F. Botrè, X. de la Torre and M. Mazzarino, Bioanalysis, 8, 1129-1132 (2016).
21
21. Y. Iwasaki, T. Sawada, K. Hatayama, A. Ohyagi, Y. Tsukuda, K. Namekawa, R. Ito, K. Saito and H. Nakazawa, Metabolites, 2, 496-515 (2012).
22
22. D. W. Armstrong and B. Zhang, Anal. Chem., 73, 577A-561A (2001).
23
23. A. N. L. Batista, F. M. dos Santos Jr., J. M. Batista Jr. and Q. B. Cass, Molecules, 23, 492-509 (2018).
24
24. F. T. Peters, O. H. Drummer and F. Musshoff, Forensic Sci. Int., 165, 216-224 (2007).
25
25. U.S. Department of Health Human Services, Food and Drug Administration, Guidance for Industry: Bioanalytical Method Validation in U.S., Beltsville, MD, 2018.
26
26. B. K. Matuszewski, M. L. Constanzer and C. M. Chavez-Eng, Anal. Chem., 75, 3019-3030 (2003).
권호 내 다른 논문
- Determination of methamphetamine and amphetamine enantiomers in human urine by chiral stationary phase liquid chromatography-tandem mass spectrometry
- Cyclodextrins’ effect on the enatioseparation of some PPIs and capillary electrophoresis method development for determining rabeprazole enantiomers
- A pilot study of a new fingerprint powder application method for the reduction of health risk
- Assessment of heavy metals in sediments of Shitalakhya River, Bangladesh
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.