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- E-ISSN 2288-8985
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A study on the analysis of artificial sweeteners in processed foods including emulsified foods by HPLC-DAD
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2020, v.33 no.5, pp.209-214
https://doi.org/10.5806/AST.2020.33.5.209
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(2020). A study on the analysis of artificial sweeteners in processed foods including emulsified foods by HPLC-DAD. Analytical Science and Technology, 33(5), 209-214, https://doi.org/10.5806/AST.2020.33.5.209
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Abstract
This study has been carried out to develop a standard method for quantifying of 3 permitted artificial sweeteners (including sodium saccharine, aspartame, acesulfame potassium) contained in foods by HPLC-DAD. A simple and rapid sample pretreatment method was used to remove fat and protein from the test solution with Carrez clearing regent precipitant know to be effective for protein and fat removal. The artificial sweeteners in the test solution purified through sample pretreatment were detected by high performance liquid chromatograph using a Reverse phase C18 column (5 μm, 4.6 × 250 mm). The simultaneous quantitative test of 3 kinds of artificial sweeteners can be effectively performed on the high fat emulsified foods containing a large amount of fat. Using the established simultaneous quantitative test method, artificial sweeteners were tested in foods such as dairy products, snacks and chocolate. The results calibration curve showed good linearity with high regression coefficients and the result of recovery test showed satisfactory recoveries within 80~110 %.
- keywords
- artificial sweeteners, simultaneous analysis, fat and emulsified food, high performance liquid chromatograph, food additive
Reference
1
1. 식품의약품안전처: 식품공전 available from http://www.mfds.go.kr/fa/index.do.2016.
2
2. S. Choi, M. Lee, E. Park, J. Won, S. Kim, S. Park and H. Lim, Korean. J. Food. Sci. Technol., 43(3), 387-395(2011).
3
3. S. Chattopadhyay, U. Raychaudhuri and R. Chakraborty, Artificial sweeteners-a review. J. Food Sci. Technol., 51, 611-621 (2014).
4
4. Food additives in food analysis contained in the MFDS, 2016.
5
5. 식품의약품안전처: 식품첨가물공전, available from http://www.mfds.go.kr/fa/index.do. 2016.
6
6. H.-Y. Kim, H.-J. Woon, K.-H. Hong, C.-H. Lee, S.-K. Park, J.-D. Choi, W.-J. Choi, S.-Y. Park, J.-H. Kim and C.-W. Lee, Korean. J. Food. Sci. Technol., 36(1), 14-18(2004).
7
7. H. J. Suh, J. Choi, D. J. An, S. Choi, D. Y. Kim and A. Y. Kim, Journal of the Korean Society of Food Science and Nutrition, 43(4), 604-611 (2014).
8
8. S.-B. Lee, K.-C. Yong, S.-L. Hwang, Y.-S. Kim, Y.-J. Jung, M.-Y. Seo, C.-H. Lee, J.-H. Sung and M.-H. Yoon, J. Fd. Hyg. Safety, 29(4), 327-333 (2014).
9
9. I.-Y. Kim, O.-J. Du, S.-D. Lee, Y.-H. Park, M.-S. Kim, C.-Ho. Bea and Y.-Z. Chae, J. Fd. Hyg. Safety, 25(2), 118-121 (2010).
10
10. S. K. Jung and M. H. Lee, “Simultaneous Determination of Artificial Sweeteners in Foods by HPLC”, 한국법과학회 학술대회, 105-106.
11
11. Foodstuffs-Determination of acesulfame-K, aspartame and saccharin-High performance liquid chromatographic method, EN method BS EN 12856:1999.
12
12. Determination of cyclamate by HPLC method, EN method BS EN 12857:1999.
13
13. J. F. Lawrence and C. F. Charbonneau, Journal of the Association of Official Analytical Chemists, 71(5), 934-937 (1988).
14
14. D. de Queiroz Pane, C. B. Dias, A. D. Meinhart, C. A. Ballus and H.T. Godoy, Journal of Food Science and Technology, 52(11), 6900-6913 (2015).
15
15. H. S. Lim, S. K. Park, I. S. Kwak, H. I. Kim, J. H. Sung, S. J. Jang and S. H. Kim, Food Science and Biotechnology, 22(1), 233-240 (2013).
16
16. A. B. Bergamo, J. A. Da Silva and D. P. De Jesus, Food Chemistry, 124(4), 1714-1717 (2011).
17
17. H. Matsumoto, K. Hirata, N. Sakamaki, K. Hagino and H. Ushiyama, Journal of the Food Hygienic Society of Japan, 49(1), 31-36 (2018).
18
18. Y. Zhu, Y. Guo, M. Ye and F. S. James, Journal of Chromatography A, 1085(1), 143-146 (2005).
19
19. Q. H. Yan, L. Yang, H. R. Zhang and L. Y. Niu, Journal of Liquid Chromatography & Related Technologies, 35(2), 268-279 (2012).
20
20. M. J. Scotter, L. Castle, D. P. T. Roberts, R. MacArthur, P. A. Brereton, S. K. Hasnip and N. Katz, Food Additives and Contaminants, 26(5), 614-622 (2009).
21
21. ISO, International Sugar Organization, Alternative sweeteners in a high sugar price environment 2012, Mark. Eval. Consum. Stat. Comm., 12:1-53.
22
22. H. Sakai, A. Yamashita, M. Tamura, A. Uyama and N. Mochizuki, Food Additives & Contaminants: Part A, 32(6), 808-816 (2015).
23
23. A. Elhassan, A. Eldean, A. Reem and A. Gomaa and M. El-Sayed, New York Science Journal, 10, 121-128(2017).
24
24. D. J. Yang and B. Chen, Food Chem., 57, 3022-3027(2009).
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