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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Spectrofluorimetric determination of Trimethoprim in pharmaceutical preparations

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2023, v.36 no.5, pp.250-257
https://doi.org/10.5806/AST.2023.36.5.250
Amneen Mohammed Alsayegh (Department of Chemistry, College of Science, University of Babylon, Hilla-51002, Iraq)
Abbas N. Alshirifi (Department of Chemistry, College of Science, University of Babylon, Hilla-51002, Iraq)
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Abstract

The development of a spectrofluorimetric method for the determination of trimethoprim according to the reaction between O-phthalaldehyde (OPA) in highly alkaline media, and 2-mercaptoethanol (2ME) and this reaction gives fluorescent product measured at (458) nm when excited at (342) nm. The optimization of the analytical parameters that influence intensity was investigated. The intensity of fluorescence of the formed product was linearly related to the concentration of trimethoprim in the (100-1200) ng mL−1 range. The limit of detection and limit of quantification were estimated to be (22.54) ng mL−1 and (75.15) ng mL−1 respectively. The utility of the proposed methods was successfully verified by analysis of trimethoprim in pure and real pharmaceutical preparations with high accuracy, the recovery percentages Re%, were found to be (100.5) % and (99.76) % for pure drug and pharmaceutical preparations respectively.

keywords
trimethoprim, O-phthalaldehyde reagent and spectrofluorimetric


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