Alkaloids of Linderae Radix suppressed the lipopolysaccharide-induced expression of cytokines in cultured macrophage RAW 264.7 cells
Kelly Yinching Lam (Hong Kong University)
Jianping Chen (Hong Kong University)
Ping Yao (Hong Kong University)
Tina Tingxia Dong (Hong Kong University)
Aizhen Xiong (Shanghai University)
Guixin Chou (Shanghai University)
Zhengtao Wang (Shanghai University)
Karl Wah-Keung Tsim (Hong Kong University)
Abstract
Linderae Radix, the dry roots of Lindera aggregata (Sims) Kosterm, has long been used as traditional Chinese medicine for treatment of inflammatory diseases. The total alkaloids are believed to be the active components responsible for anti-inflammation of Linderae Radix. Here, the total alkaloids of Linderae Radix were extracted and isolated, including 12 isoquinoline alkaloids and 1 furan sesquiterpene. Within the alkaloids, norisoboldine, boldine, linderaline, isoboldine, reticuline, N-methyllaurotetanine, norjuziphine were found to be the major ingredients. In lipopolysaccharide-treated macrophage RAW 264.7 cells, application of Linderae Radix extract, or total alkaloids, suppressed the transcription of pro-inflammatory cytokines, interleukin-1β and interleukin-6. Out of the 12 alkaloids, norisoboldine, boldine, and isoboldine were tested in lipopolysaccharide-treated macrophages, and norisoboldine was the strongest alkaloid in suppressing the cytokine expressions. The current studies suggested that the identification of alkaloids from Linderae Radix could provide a plausible explanation for herbal therapeutic functions.
- keywords
- traditional Chinese medicine, Linderae Radix, total alkaloids, isoquinoline alkaloids, pro-inflammatory cytokines
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