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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects of Woohwangcheongsim-won on the Gene Expression in a Hypoxic Model of Cultured Rat Cortical Cells

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.3, pp.123-136






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Abstract

Objective: The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won(WC) on the in vitro neuronal development and alteration in gene expression in a hypoxia model using cultured rat cortical cells.Methods: E18 rat cortical cells were grown in neurobasal medium containing B27 supplement and various concentration of WC. Initial development of growth cone was investigated by phase-contrast microscopy, while dendritic spine formation and synaptogenesis were investigated by immunocytochemistry with SynGAPα (a postsynaptic marker) and synaptophysin (presynaptic marker) antibodies. Alteration in gene expression was analyses by microarray using rat 5K-TwinChips.Results: WC suppressed the development of growth cones and WC increased the number of dendritic spines at 20 and 50㎍/mL concentration but there was no statistical significance. Instead, it significantly decreased the number at 100㎍/mL. The expression of anti-apoptosis gene Bcl2-like 1(Bcl2l1) was increased (Global M=0.46), while Akt1 was decreased. Proapoptosis genes Bad and PDCD2 was increased. The expression of hemoglobin alpha 1(probably neuroglobin) was increased (Global M=0.93). The exprssion of antioxidants such as catalase, heme oxigenase(HO), PRKAG2 gene was increased. The expression PKC gene was increased. The expression of retinoic acid receptor alpha (RARα) was significantly increased (Global M=1.0).Conclusion: These data suggest that WC trends to supress cellular activity slightly in normoxia and increases the expression of apoptosis-, antioxidation-, oxygen capture-related genes in hypoxia, but increases Bcl1l1 that anti-apoptosis gene, on the other hand increases Bad, PDCD2 that pro-apoptosis genes, too.

keywords
Woohwangcheongsim-won(WC), gene expression, microarray, dendritic spine, delayed neuronal death, hypoxia, Woohwangcheongsim-won(WC), gene expression, microarray, dendritic spine, delayed neuronal death, hypoxia


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