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- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Fingerprint of Marker Substances in Gami-Honghwa-Tang(KH-19) by HPLC-DAD
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.3, pp.45-54
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(2004). Fingerprint of Marker Substances in Gami-Honghwa-Tang(KH-19) by HPLC-DAD. Journal of Korean Medicine, 25(3), 45-54.
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Abstract
Objective: This study was aimed to evaluate marker substances in Gami-Honghwa-Tang(KH-19) by high performance liquid chromatography-photodiode array detector (HPLC-DAD). Gami-Honghwa-Tang is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos and Glycyrrhizae Radix. Methods: The separation was performed on a Aquasil C18 (4.6X 250mm) column by gradient elution with 0.05% TFA in H2O - 0.05% TFA in acetonitrile (0min 100:0, 20min, 90:10, 40min 70:30, 60min 50:50, 80min 0:100, 90min 100:0) as the moblie phase at a flow-rate of 1.0ml/min with detection at 190-800nm. Also we examined the contents of bacteria, residue of pesticide and harmful heavy metal. Results: HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, decursin in the KH-19. And there was no contents of bacteria, residue of pesticide, and harmful heavy metal.Conclusion: We suggest this results could be a useful evidence for quality control of KH-19. This method permits fingerprint of selected individual marker substances from herbal prescriptions without derivatization, multiple purification steps, or lengthy separation times.
- keywords
- Gami-Honghwa-Tang, HPLC-DAD, marker substance, fingerprint., Gami-Honghwa-Tang, HPLC-DAD, marker substance, fingerprint.
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