- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Fingerprint of Marker Substances in Gami-Honghwa-Tang(KH-19) by HPLC-DAD
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.3, pp.45-54
&
(2004). Fingerprint of Marker Substances in Gami-Honghwa-Tang(KH-19) by HPLC-DAD. Journal of Korean Medicine, 25(3), 45-54.
- Downloaded
- Viewed
Abstract
Objective: This study was aimed to evaluate marker substances in Gami-Honghwa-Tang(KH-19) by high performance liquid chromatography-photodiode array detector (HPLC-DAD). Gami-Honghwa-Tang is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos and Glycyrrhizae Radix. Methods: The separation was performed on a Aquasil C18 (4.6X 250mm) column by gradient elution with 0.05% TFA in H2O - 0.05% TFA in acetonitrile (0min 100:0, 20min, 90:10, 40min 70:30, 60min 50:50, 80min 0:100, 90min 100:0) as the moblie phase at a flow-rate of 1.0ml/min with detection at 190-800nm. Also we examined the contents of bacteria, residue of pesticide and harmful heavy metal. Results: HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, decursin in the KH-19. And there was no contents of bacteria, residue of pesticide, and harmful heavy metal.Conclusion: We suggest this results could be a useful evidence for quality control of KH-19. This method permits fingerprint of selected individual marker substances from herbal prescriptions without derivatization, multiple purification steps, or lengthy separation times.
- keywords
- Gami-Honghwa-Tang, HPLC-DAD, marker substance, fingerprint., Gami-Honghwa-Tang, HPLC-DAD, marker substance, fingerprint.
Reference
1
日本防菌防黴學會, (1999) 21世紀의 生藥 漢方製劑, 纖維社.
2
식품의약품안전청, (2002) 대한약전 제 8개정. ,
3
식품의약품안전청, (2004) 대한약전외한약(생약)규격집,
4
조선민주주의인민공화국 보건부 약전위원회, (1996) 조선민주주의인민공화국 약전, 의과학출판사
5
일본약국방해설서 편집위원회, (2001) 일본약국방해설서, 광천서림
6
국가약전위원회, (2000) 중화인민공화국약전, 화학공업출판사
7
WHO, (1998) WHO monographs on selected medicinal plants, WHO
8
療用漢方엑기스製의品質評 - 3次元HPLC fingerprint技術의用 - 쯔무라 기술보고서 21,
9
服部子他, (2002) 第19回和漢要旨집, 일본화한의약학회
10
Saiki I,, (1999) HPLC analysis of juzen-taiho-to and its variant formulations and their antimetastatic efficacies,
11
Yu Y.B.,, (2000) Evaluation on the Safety of gamma-irradiated Angelica gigas Nakai Stability of Active components and safety in genotoxicity test,
12
He JX,, (2002) Development of a simple HPLC method for determination of paeoniflorin-metabolizing activity of intestinal bacteria in rat feces,
13
Lee J.H.,, (2002) Quantitative Determination of 5-Hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to Various Processings,
14
Wang SF,, (2003) Analysis of three effective components in Fructus corni and its preparations by micellar electrokinetic capillary chromatography,
15
Zhao X, (2003) Analysis of Paeoniae Radix by high-performance liquid chromatography-electrospray ionization-mass spectrometry,
16
Jiang Y,, (2004) Preparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography,
17
식품의약품안전청, (2001) 생약의 잔류농약 허용기준 및 시험 방법,
18
식품의약품안전청, (2001) 생약 등의 중금속허용기준 및 시험방법,
19
Haghighat Khajavi S,, (2004) Decomposition kinetics of maltose in subcritical water,
20
Hanai T, (1999) Cambridge: Royal Society of Chemistry,
21
Ahuja S, (1989) Selectivity and detectability optimizations in HPLC, Wiley
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- The Defensive Effect of Samooltang on Injury of Gastric Mucous Membrane of Mouse by Ethanol
- Grope for a Summary Program about Traditional Medical Database for Intellectual Property Protection in WIPO
- Grope for a Summary Program about Intellectual Property Protection of Traditional Knowledge (TK)etc. Discussed in WIPO
- Study on the Effect of Gamisihotang(GSHT) on Gultamate Receptor, Free Radical and Brain Damage in Rats Subjected to Brain Ischemia
- Fingerprint of Marker Substances in Gami-Honghwa-Tang(KH-19) by HPLC-DAD
- Effect of Atractylodis Macrocephalae Rhizoma on Starvation Stress in Mice
- α2-Adrenoceptors are？ Implicated in the Electroacupuncture-induced Analgesia in the Experimental Chronic Pain
- A Review on the Report about Drug-induced Hepatitis published by National Institute of Toxicological Research
- Inhibition of COX-2 gene expression via NF-κB pathway by Ichungwhan
- The Clinical Study on the Live Blood Condition of Women's Shoulder Measurement
- Genetic Polymorphism of CCK Promotor Region and Sasang Constitution
- Effects of Jaeumgeonbi-tang Extract on Indomethacin-Induced Gastric Mucosal Lesions.
- Effects of Woohwangcheongsim-won on the Gene Expression in a Hypoxic Model of Cultured Rat Cortical Cells
- The Efficacy of Sihocheonggan-san(Chaihuqinggan-san) Extract to Improve he Function of Stratum Corneum on Mice Model after Atopic Dermatitis Elicitation
- Protective Effects of Gyungokgo on Oxidative Stress-Induced Apoptosis of H9c2 Cardiomyoblast Cells
- Therapeutic Effects of Extract of？ Uncariae Ramulis and Testudinis Plastrum on Cochlear Morphologic Change Induced by Salicylate Ototoxicity
- The Review on the Study of Diabetes Mellitus in the Oriental Medicine Journal
- A study for biocompatibility of acupuncture's metal material -focused on biological character-
- Antitumor Effect of Gagamjengac-tang and As2O3 in Human Lung Cancer Cell Line, H-157
- A Case report of Seupon(Shiwen,濕溫) with Gyeonggwol(Jingjue,痙厥)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.