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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Inhibition of COX-2 gene expression via NF-κB pathway by Ichungwhan

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2004, v.25 no.3, pp.90-98


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Abstract

Objectives : The present study was undertaken to investigate the molecular mechanisms of Ichungwhan for inhibition of cyclooxygenase-2 (COX-2) gene expression via suppression of NF-κB (nuclear factor κB) using aged-rat. NF-κB is the most important modulator of inflammation and NF-κB regulates the gene expression of several pro-inflammatory cytokines, such as COX-2. Methods : In the experiment, we investigated the scavenging property of Ichungwhan on reactive species (RS) including nitrogen-derived species (RNS) was measured by DCF-DA (2,7-Dichlorodihydrofluorexcein diaceetate) / DHR 123 (Dihydrorhodamine 123) assay. Protein expression levels of COX-2, NF-κB, p-ERK and p-p38 were assayed by western blot. Results : We investigated that Ichungwhan inhibit RS including RNS and Ichungwhan inhibit NF-κB activation by blocking the dissociation of inhibitory IκB-β via suppression of IKK pathway. Also Ichungwhan inhibit COX-2 gene expression. Conclusions : These findings suggest that Ichungwhan modulates COX-2 gene expression via suppression of NF-κB pathway.

keywords
peroxynitrite, reactive species, cyclooxygenase-2, nuclear factor κB, Ichungwhan, peroxynitrite, reactive species, cyclooxygenase-2, nuclear factor κB, Ichungwhan


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