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Determination of Putrescine, Spermidine and Spermine in urine by HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.6, pp.445-452
Shin,
T., Lee,
S., &
Kim,
H.
(1997). Determination of Putrescine, Spermidine and Spermine in urine by HPLC. Analytical Science and Technology, 10(6), 445-452.
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Abstract
A simple and sensitive high performance liquid chromatography for analysis of Putrescine, Spermidine and Spermine in urine is described. The benzoyl chloride derivatives of Putrescine, Spermidine and Spermine are separated on a <TEX>${\mu}$</TEX> Bondapak <TEX>$C_{18}$</TEX> reverse-phase column with methanol/<TEX>$H_2O$</TEX>(50/50v/v) as the mobile phase. The results show that the mean level of polyamines in uterine cancer patients urine is much higher than in normal human urine.
- keywords
- HPLC analysis, Putrescine, Spermidine, Spermine, Benzoyl chloride
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