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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Fundamental Investigation of Non-invasive Determination of Alcohol in Blood by Near Infrared Spectrophotometry

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.5, pp.375-381
Chang, Soo-Hyun
Cho, Chang-Hee
Woo, Young-Ah
Kim, Hyo-Jin
Kim, Young-Man
Lee, Kang-Boong
Kim, Young-Woon
Park, Sung-Woo
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Abstract

Near infrared spectrophotometry(NIR) was developed as a non-invasive determination of blood alcohol. The first pure alcohol/water samples were prepared with ethanol concentration from 0.01 to 0.1%(w/w). Analysis of the second-derivative data was accomplished with multilinear regression(MLR). The standard error of calibration(SEC) of ethanol in ethanol/water solutions was approximately 0.0039%. The calibration models were established from the blood alcohol spectra by MLR and PLSR analysis. The best calibration was built with the second-derivative spectra of 2266 and 2326 nm by MLR. Second-derivative spectra in the spectral ranges of 1100~1340, 1500~1796 and 2064~2300 nm with four PLSR factors provided the standard error of prediction(SEP) of 0.030%(w/w). These results indicate that NIR may be applied for a fast non-invasive determination of alcohol in the blood.

keywords
near infrared spectrophotomeuy(NIR), non-invasive determination, alcohol, multiple linear regression(MLR), panial least squares regression(PLSR)


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