ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

전기화학적 방법에 의한 HRP의 최적 pH도출

Electrochemical Determination of the Optimum pH of HRP

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.6, pp.504-508
윤길중 (청주대학교)
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Abstract

A carbon paste electrode was constructed with peroxidase extracted from Horseradish and the variation of the response of the sensor with pH was investigated. Current profiles showed two highest sensitivities at two pH values respectively. In addition, two bands were observed in the electrophoretic expansion. A coincidence of the two experimental results added support to the possibility that the biosensor has two different isozymes. Assuming that current profiles are the sum of two gaussians, we deconvoluted them and determined the optimum pH of peroxidase isozymes.

keywords
biosensor, carbon paste electrode, isozyme, peroxidase


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