• P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

전기화학적 방법에 의한 HRP의 최적 pH도출

Electrochemical Determination of the Optimum pH of HRP

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.6, pp.504-508
윤길중 (청주대학교)
  • 76다운로드 수
  • 263조회수

Abstract

A carbon paste electrode was constructed with peroxidase extracted from Horseradish and the variation of the response of the sensor with pH was investigated. Current profiles showed two highest sensitivities at two pH values respectively. In addition, two bands were observed in the electrophoretic expansion. A coincidence of the two experimental results added support to the possibility that the biosensor has two different isozymes. Assuming that current profiles are the sum of two gaussians, we deconvoluted them and determined the optimum pH of peroxidase isozymes.

keywords
biosensor, carbon paste electrode, isozyme, peroxidase


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