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Electrochemical Determination of the Optimum pH of HRP
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.6, pp.504-508
(2003). Electrochemical Determination of the Optimum pH of HRP. Analytical Science and Technology, 16(6), 504-508.
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Abstract
A carbon paste electrode was constructed with peroxidase extracted from Horseradish and the variation of the response of the sensor with pH was investigated. Current profiles showed two highest sensitivities at two pH values respectively. In addition, two bands were observed in the electrophoretic expansion. A coincidence of the two experimental results added support to the possibility that the biosensor has two different isozymes. Assuming that current profiles are the sum of two gaussians, we deconvoluted them and determined the optimum pH of peroxidase isozymes.
- keywords
- biosensor, carbon paste electrode, isozyme, peroxidase
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