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- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Identification of human blood using Rapid FOB (Fecal Occult Blood) Test Kit
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.3, pp.211-216
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(2004). Identification of human blood using Rapid FOB (Fecal Occult Blood) Test Kit. Analytical Science and Technology, 17(3), 211-216.
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Abstract
Commercial one-step rapid fecal occult blood (FOB) kit which was used as a screening test to detect traces of blood in stool samples was evaluated for the feasibility of the forensic identification of human blood. The sensitivity was determined and compared with the conventional Leucomalichite green (LMG) method. In addition, the specificity of the kit and the effects of various chemicals and environmental factors were examined. FOB kit was specific for human hemoglobin and more sensitive than LMG test (approximately 100 times). FOB kit showed positive band using at least 1,000,000-fold diluted human blood. The antigen was very stable regardless of storage temperature and boiling. The positive reaction was not affected by LMG and Luminol, the traditional tests for identification of bloodstain. As a results, FOB test kit could be effectively applied to identification of human blood at crime scene and crime laboratories.
- keywords
- Blood stain, FOB kit, LMG, Luminol, Sensitivity, Human-specificity
Reference
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(2001) Criminalistics: An Introduction to Forensic Sciences, Prentice Hall
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