- P-ISSN 1225-0163
- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Quantitation of L-carnitine in plasma by electrospray ionization tandem mass spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2005, v.18 no.2, pp.163-167
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(2005). Quantitation of L-carnitine in plasma by electrospray ionization tandem mass spectrometry. Analytical Science and Technology, 18(2), 163-167.
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Abstract
In this study, a novel analytical method has been developed for the rapid determination of L-carnitine in human plasma using electrospray ionization tandem mass spectrometry. Free carnitine (FC) was analyzed after extraction with 80% methanol and total carnitine (TC) was analyzed after hydrolysis and extraction. Acyl carnitine (AC) was subtracted FC from TC. Analytical methods used multiple reaction monitoring (MRM) scan modes. A correlation coefficient of linear regression (r2) was 0.9995, recovery was 97%, reproducibility was less than 10%, and limit of detection (LOD) was 0.0016 μmol/L. This method reduced sample preparation time and showed high resolution and good reproducibility compared to that with liquid chromatographic methods. Normal control showed AC was lower than FC. Clinical management of patients with inborn error of metabolism showed FC was lower than AC. Thus, carnitine fraction level was very important to monitoring patients with metabolic disorder
- keywords
- IR, NMR, MMPs, Zymography, SK-Hept-1, MTT
Reference
1
R. A Chalmers, (1982.) rganic acid in man,
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