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- E-ISSN 2288-8985
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Planar microchip-based lactate biosensor
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2006, v.19 no.6, pp.482-489
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(2006). Planar microchip-based lactate biosensor. Analytical Science and Technology, 19(6), 482-489.
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Abstract
Two electrode-based lactate biosensor was prepared by immobilizing lactate oxidase (LOD) obtained from pediococcus species in a poly(vinyl alcohol). Hydrogen peroxide (<TEX>$H_2O_2$</TEX>) produced by the reaction of lactate and LOD was detected on the Pt-black that was electrochemically deposited on the Au electrode. Sensors fabricated with Pt-black deposited Au electrode provided a high current of <TEX>$H_2O_2$</TEX> oxidation at a substantially lowered applied potential (+300 mV vs. Ag/AgCl), resulting in reduced interferences from easily oxidizable species such as ascorbic acid, acetaminophen, and uric acid. An outer membrane is formulated by adjusting water uptake of hydrophilic polyurethane (HPU). The sensor performance was evaluated in vitro with both flow-through arrangement and static mode. The sensor showed a linear range from 0.1 mM to about 9.0 mM in 0.05 M phosphate buffer (pH 7.6) containing 0.05 M NaCl. Storing the sensors prepared in this work at <TEX>$4^{\circ}C$</TEX> buffer solution while not in use, they provided same electrochemical performance for more than 25 days.
- keywords
- planar microchip, lactate, lactate oxidase, hydrophilic polyurethane
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