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- E-ISSN 2288-8985
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The enhancement of protein separation by duplex SDS-PAGE
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2006, v.19 no.6, pp.529-534
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(2006). The enhancement of protein separation by duplex SDS-PAGE. Analytical Science and Technology, 19(6), 529-534.
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Abstract
The protein separation with molecular weight using SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is the one of the most conventional and simple techniques. In, this study, two dimensional SDS-PAGE using same separation principle consecutively was investigated and compared with one dimensional SDS-PAGE. The enhanced separation from duplex SDS-PAGE was observed and separated proteins in the gel were identified by MALDI TOF MS. Identified proteins from different gel spots were found to have different gi numbers. Therefore, duplex SDS-PAGE separation method will be used for economic separation method in the future because only tiny amount of inexpensive reagents are used to perform duplex SDS-PAGE.
- keywords
- protein separation, SDS-PAGE, MALDI TOF MS
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