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- E-ISSN 2288-8985
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Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP - HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2008, v.21 no.2, pp.102-108
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(2008). Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP - HPLC. Analytical Science and Technology, 21(2), 102-108.
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Abstract
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0- 8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
- keywords
- liquiritin, glycyrrhizic acid, glabridin, licorice, RP-HPLC
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