- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Primary study of sterols composition of Rhodiola sachalinensis by using GC/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2009, v.22 no.3, pp.219-227
&
(2009). Primary study of sterols composition of Rhodiola sachalinensis by using GC/MS. Analytical Science and Technology, 22(3), 219-227.
- Downloaded
- Viewed
Abstract
The steroid compounds in Rhodiola sachalinensis were determined with adsorption column chromatographic purification and GC/MS. Sterols were extracted by sonication and Soxhlet with ethanol and dichloromethane, respectively. The extract was partitioned with chloroform and water using liquid-liquid extraction, and purified with a silica column after the sterols had been converted to the corresponding silyl derivatives with BSTFA. Eighteen free sterols, including β-sitosterol, stigmasterol and cycloartenol, and nine sterol conjugates were found from Rhodiola sachalinensis by GC/MS. Among them, cholest-5-ene-3-ol, cholesterol, stigmasterol, β-sitosterol were confirmed and quantified with sterol standards. Most sterols were presented in the chloroform part, with C29 being the most abundant group in this sterol group. β-sitosterol was the most abundant compound with a relative content of 45.94% followed by ergost-7-ene-3-ol (11.33%), 4,14-dimethyl-ergosta-8,24(28)-diene-3-ol (7.07%), stigmasterol (6.09%), cycloartenol (5.43%) and 4-methyl-cholest-5-ene-3-ol (5.39%).
- keywords
- Rhodiola sachalinensis, Sterols, Derivatives, Sonication, Soxhlet, GC/MS
Reference
1
1. G. Zhou, Y. Guan, H. Chen and J. Ye, J. Chromatogr. A., 1142, 236-239(2007).
2
2. S. Nakamura, X. Li, H. Matsuda, K. Ninomiya, T. Morikawa, K. Yamaguti and M. Yoshikawa, Chem. Pharm. Bull., 55, 1505-1511(2007).
3
3. C. Y. Ma, J. Tang, H. Wang, X. Gu and G. Tao, Chromatographia, 67, 383-388(2008).
4
4. Y. Mao, Y. Li and N. Yao, J. Pharm. & Biomed. Anal., 45, 510-515(2007).
5
5. J. X. Nan, Y. Z. Jiang, E. J. Park, G. Ko, Y. C. Kim and D. H. Sohn, J. Ethnopharm., 84, 143-148(2003).
6
6. S. Zhang, H. Bi and C. Liu, Sep. Purif. Technol., 57, 277-282(2007).
7
7. C. R. Salma, B. Souhail, L. Georges, B. Christophe, D. Claude and A. Hamadi, J. Food. Compos. Anal., 21, 162-168(2008).
8
8. S. Apparich and F. Ulberth, J. Chromatogr. A., 1005, 169-176(2005).
9
9. S. S. Cunha, J. O. Fernandes and M. B. P. P. Oliveira, J. Chromatogra. A., 1128, 220-227(2006).
10
10. M. J. Lagarda, G. Garcia-Llatas and R. Farré, J. Pharm. Biomed. Anal., 41, 1486-1496(2006).
11
11. T. Li, G. Xu, L. Wu and C. Sun, Phytomedicine, 14, 601-604(2007).
12
12. D. Li, J. Park and J. R. Oh, Anal. Chem., 73, 3089-3095 (2001).
13
13. D. Li, M. Dong, W. J. Shim and N. Kannan, J. Chrmatogr. A., 1160, 64-70(2007).
14
14. A. Mohammad and R. Gupta, Colloids and Surfaces B: Biointerfaces, 65, 166-171(2008).
15
15. S. Gunawan, N. S. Kasim and Y. H. Ju, Sep. Purif. Tech., 60, 128-135(2008).
16
16. C. Jiang, M. Li and A. C. T. Duin, Organic Geochemistry, 31, 751-756(2000).
17
17. T. Akihisa, T. Gebreyesus, H. Hayashi and T. Tamura, Phytochemistry, 31, 163-166(1992).
18
18. M. Kalinowska, W. R. Nes, F.G. Crumley and W. D. Nes, Phytochemistry, 29, 3427-3434(1990).
19
19. K. N. Gaind, A. K. Singla, R. B. Boar and D. B. Copsey, Phytochemistry, 15, 1999-2000(1976).
20
20. J. P. Bianchini, A. Ralaimanarivo, E. M. Gaydou and B. Waegell, Phytochemistry, 21, 1981-1987(1982).
21
21. A. Lisa, Acta Universitatis Agriculturae Sueciae, 128, (2007).
22
22. P. Benveniste, American Society of Plant Biologists, 1- 31 (2002).
23
23. T. Akihisa, W. C. M. C. Kokke, T. Tamura and T. Matsumoto, Lipids, 26, 660-665(1991).
Other articles from this issue
- Study on establishment of emission cell test method for liquid phase building materials
- Monitoring of some heavy metals in oriental animality medicines
- Simultaneous determination of amphetamine derivatives and norketamine in hair by GC-MS/MS
- Primary study of sterols composition of Rhodiola sachalinensis by using GC/MS
- Analysis on the substrate specificity and inhibition effect of Brassica oleracea glutathione S-Transferase
- Trace element analysis of korean car windshield using LA-ICP-MS
- The development of encoded porous silicon nanoparticles and application to forensic purpose
- Fabrication of Fe-ACF/TiO2 composites and their photonic activity for organic dye
- A comparative study on consolidants for bronze objects conservation
- Generation characteristics of disinfection by-products (DBPs) by chlorination in sewage effluent
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.