- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Apply for Authority
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Development of chemical ionization method in a GC-TOF mass spectrometer for accurate mass and isotope ratio measurement
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.1, pp.15-23
https://doi.org/10.5806/AST.2011.24.1.015
&
(2011). Development of chemical ionization method in a GC-TOF mass spectrometer for accurate mass and isotope ratio measurement. Analytical Science and Technology, 24(1), 15-23, https://doi.org/10.5806/AST.2011.24.1.015
- Downloaded
- Viewed
Abstract
An accurate mass and isotope ratio were determined using a gas chromatography/time of flight mass spectrometer in CI positive mode for the identification of unknown metabolites. High mass tune was used to improve the ion intensity of [M+H]^+. Chromatographic resolution and dynamic range enhancement were performed to obtain more reliable accurate masses and correct isotope abundance ratios. Average absolute errors of mass and isotope ratios for 24 reference metabolite -TMS (trimethylsilyl) derivatives were 6.8 ppm, 1.5%of (M+1/M ratio) and 1.7% of (M+2/M ratio), respectively. The correct formulas of twenty one compound were retrieved within top-2 hit from the heuristic algorithm for elemental composition using each accurate mass and isotope abundance ratio.
- keywords
- accurate mass, isotope ratio, GC-TOF/MS, positive CI, metabolites
Reference
1
1. G. V. Silas, S. Mas, M. Kesson, J. Smedsgaard and J. Nielsen, Mass Spectrometry Reviews, 24, 613-646(2005).
2
2. http://www.nist.gov/srd/nist1a.cfm
3
3. O. Fiehn, J. Kopka, P. Drmann, T. Altmann, R. N. Trethewey and L. Willmitzer, Nat. Biotechnol. 18, 1157-1161(2000).
4
4. S. Strelkov, M. V. Elstermann and D. Schomburg, Bio. Chem., 385, 853-861(2004).
5
5. C. Denkert, J. Budczies, T. Kind, W. Weichert, P. Tablack, J. Sehouli, S. Niesporek, D. Koensgen, M. Dietel and O. Fiehn, Cancer Res., 66(22), 10795-10804(2006).
6
6. J. Lisec, N. Schauer, J. Kopka, L. Willmitzer and A. R. Fernie, Nature Protocols 1, 387-396(2006).
7
7. D. Y. Lee and O. Fiehn, Plant Methods, 4, 1-13(2008).
8
8. D. Herebian and B. Hanisch, F.-J. Marner, Metabolomics, 1(4), 317-324(2005).
9
9. V. V. Tolstikov, Methods Mol. Biol., 544, 343-353(2009).
10
10. A. Gordin, A. B. Fialkov and A. Amirav, Rapid Commun Mass Spectrom., 22(17), 2660-2666(2008).
11
11. R. Flamini, A. D. Vedova, D. Cancian, A. Panighel and M. D. Rosso, J. Mass Spectrom., 42(5), 641-646(2007).
12
12. Q. Kuangnan, D. J. Gary and E. E. Kathleen, Int. J. Mass Spectrom., 265, 230-236(2007).
13
13. R. C. Jose and P. R. Paul, Organic Geochemistry, 30, 279-286(1999).
14
14. W. Welthagen, S. Mitschke, F. Mühlberger and R. Zimmermann, J. Chromatogr. A., 1150, 54-61(2007).
15
15. Z. Ralf, W. Werner and G. Thomas, J. Chromatogr. A., 1184, 296-308(2008).
16
16. T. Kind and O. Fiehn, BMC Bioinformatics, 7, 234, 1- 10(2006).
17
17. T. Kind and O. Fiehn, BMC Bioinformatics, 8, 115, 1- 20(2007).
18
18. Y. Wang and M. Gu, Anal Chem., 82(17), 7055-62(2010).
19
19. J. C. Erve, M. Gu, Y. Wang, W. DeMaio and R. E. Talaat, J. Am. Soc. Mass. Spectrom., 20(11) 2058-69(2009).
20
20. M. Gu, Y. Wang, X. G. Zhao and Z. M. Gu, Rapid Commun Mass Spectrom., 20(5), 764-70(2006).
21
21. http://fiehnlab.ucdavis.edu/projects/Seven_Golden_Rules/
22
22. T. Kind, G. Wohlgemuth, D. Y. Lee, Y. Lu, M. Palazoglu, S. Shahbaz and O. Fiehn, Anal. Chem., 81(24), 10038-10048(2009).
23
23. O. Masaki, Y. Hiroyuki, F. Hirohiko, K. Satoshi, Y. Daisuke, O. K. Koji, M. Eizi, S. Kazuhiko and U. Nobuo, Nucl. Instr. and Meth. in Phys. Res. B 236(1) 377-382(2005).
24
24. T. Kind, V. V. Tolstikov, O. Fiehn and R. H. Weiss, Anal. Biochem., 363, 185-195(2007).
25
25. S. Abate, Y. G. Ahn,T. Kind, T. Cataldi and O. Fiehn, Rapid Commun Mass Spectrom., 24, 1172-1180(2010).
Other articles from this issue
- Uncertainty evaluation of the analysis of 11-nor-9-carboxy-Δ^9-tetrahydrocannabinol in hair by GC-NCI-MS/MS
- Determination of bromide in bottled mineral water and ground water in Korea
- Development of chemical ionization method in a GC-TOF mass spectrometer for accurate mass and isotope ratio measurement
- Ionic composition comparison of atmospheric aerosols at coastal and mountainous sites of jeju island
- Effects of the decoction water on the extraction of the bioactive compounds from rhubarb
- A study on characteristics of latent fingerprint detection on vinyl leather
- Research on the detection of LCN DNA from traces on firearms
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.