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- E-ISSN 2288-8985
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Analysis of dutasteride in human serum by LC-MS/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2012, v.25 no.1, pp.76-82
https://doi.org/10.5806/AST.2012.25.1.076
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(2012). Analysis of dutasteride in human serum by LC-MS/MS. Analytical Science and Technology, 25(1), 76-82, https://doi.org/10.5806/AST.2012.25.1.076
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Abstract
The determination and confirmation of dutasteride in human serum was performed by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Beclomethasone as an internal standard (I.S.) was added to the serum and the mixed sample was pretreated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The mass transitions of dutasteride and I.S. monitored in multiple reaction monitoring (MRM) were m/z 529.6→461.5 and m/z 409.3→391.2, respectively, and the retention times were 6.45 and 5.46min, respectively. The calibration curve was linear in the concentration range of 0.5~30.0 ng/mL (R2= 0.9999) and the limit of quantitation (LOQ) was found to be 0.5 ng/mL. The recovery of dutasteride was shown to be 66~72%. The intra-day assay precision and accuracy were in the range 3.5~5.5% and 85.7~89.9%, respectively, and the interday assay precision and accuracy were in the range 4.2~5.8% and 90.8~95.8%, respectively
- keywords
- Dutasteride, LC-MS/MS, human serum
Reference
1
1. C. G. Roehrborn, P. Boyle, J. C. Nickel, K. Hoefner and G. Andriole, Urol., 60, 434-441 (2002).
2
2. H. C. Evans and K. L. Goa, Drugs Aging., 20(12), 905-916 (2003).
3
3. K. K. Gaines, Urol. Nurs., 23(3), 218-220 (2003).
4
4. G. L. Andriole and R. Kirby, Eur. Urol., 44, 82-88(2003).
5
5. F. Debruyne, J. Barkin, P. van Erps, M. Reis, T. L. J. Tammela and C. Roehrborn, Eur. Urol., 46(4), 488-495(2004).
6
6. R. V. Clark, D. J. Hermann, G. R. Cunningham, T. H. Wilson, B. B. Morrill and S. Hobbs, J. Clin. Endocrinol Metab., 89(5), 2179-1284 (2004).
7
7. E. A. Olsen, M. Hordinskym, D. Whiting, D. Stough, S. Hobbs, M. L. Ellis, T. Wilson and R. S. Rittmaster, J. AM ACAD DERMATOL., 55(6), 1014-1026 (2006).
8
8. M. Marberger, C. G. Roehrborn, L. S. Marks, T. Wilson and R. S. Rittmaster, J. Clin. Endocrinol. Metab., 91(4), 1323-1328 (2006).
9
9. C. G. Roehrborn, P. Siami, J. Barkin, R. Damião, K. Major-Walker, B. Morrill and F. Montorsi, CombAT Study Group, J. Urol., 179(2), 616-621 (2008).
10
10. J. C. Nickel, Urol., 6(9), S31-39 (2004).
11
11. N. Makridakis and J. K. V. Reichardt, J. Mol. Endocrinol., 34, 617-623 (2005).
12
12. J. K. Amory, C. Wang, R. S. Swerdloff, B. D. Anawalt, A. M. Matsumoto, W. J. Bremner, S. E. Walker, L. J. Haberer and R. V. Clark, J. Clin. Endocrinol. Metab., 92(5), 1659-1665 (2007).
13
13. C. L. Foley, S. R. Bott, I. S. Shergill and R. S. Kirby, Drugs. Today., 40(3), 213 (2004).
14
14. A. M. Traish, J. Hassani, A. T. Guay, M. Zitzmann and M. L. Hansen, J. Sexual. Medicine., 8(3), 872-884 (2011).
15
15. Blood Guidances, Food and Drug Administration, USA, 2011.
16
16. Blood Regulation Law, Ministry of Health and Welfare, Korea, 2011.
17
17. N. V. S. Ramakrishna, K. N. Vishwottam, S. Puran, M. Koteshwara, S. Manoj and M. Santosh, J. Chromatogr B., 809(1), 117-124 (2004).
18
18. S. Agarwal, K. V. Gowda, A. K. Sarkar, D. Ghosh, U. Bhaumik, T. K. Chattarai and T. K. Pal, Chromatographia., 67, 893-903 (2008).
19
19. N. A. Gomes, A. Pudage, S. S. Joshi, V. V. Vaidya, S. A. Parekh and A. V. Tamhankar, Chromatographia., 69, 9-18 (2009).
20
20. Bioanalytical Method Validation, National Institute of Food and Drug Safety Evaluation, Korea, 2009.
21
21. Guidance for Industry, Bioanalytical Method Validation, Food and Drug Administration, USA, 2001.
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